ELISA/assay

Rat Relaxin ELISA Kit

MBS723992

48-Strip-Wells

470 USD

ELISA Kit

Rat Relaxin ELISA Kit

Relaxin

relaxin; Prorelaxin H1; prorelaxin H1; preprorelaxin H1; relaxin 1

RLT

RLN1; RLN1; H1; H1RLX; RLXH1; bA12D24.3.1; bA12D24.3.2

REL1_HUMAN

Rat

This assay has high sensitivity and excellent specificity for detection of RLT. No significant cross-reactivity or interference between RLT and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between RLT and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 pg/mL.

Intended Uses: This RLT ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat RLT. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Signal Transduction

Principle of the assay: RLT ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-RLT antibody and an RLT-HRP conjugate. The assay sample and buffer are incubated together with RLT-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the RLT concentration since RLT from samples and RLT-HRP conjugate compete for the anti-RLT antibody binding site. Since the number of sites is limited, as more sites are occupied by RLT from the sample, fewer sites are left to bind RLT-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The RLT concentration in each sample is interpolated from this standard curve.

490056

CAA00599.1

13,011 Da

Androgen Receptor Signaling Pathway (198806); Myometrial Relaxation And Contraction Pathways (198759)

Relaxins are known endocrine and autocrine/paracrine hormones, belonging to the insulin gene superfamily. In humans there are three non-allelic relaxin genes, RLN1, RLN2 and RLN3, where RLN1 and RLN2 share high sequence homology. The protein encoded by this gene is synthesized as a single-chain polypeptide but the active form consists of an A chain and a B chain linked by disulfide bonds. Relaxin is produced by the ovary, and targets the mammalian reproductive system to ripen the cervix, elongate the pubic symphysis and inhibit uterine contraction. It may have additional roles in enhancing sperm motility, regulating blood pressure, controlling heart rate and releasing oxytocin and vasopressin. [provided by RefSeq, Jan 2013]

RLN1: Relaxin is an ovarian hormone that acts with estrogen to produce dilatation of the birth canal in many mammals. May be involved in remodeling of connective tissues during pregnancy, promoting growth of pubic ligaments and ripening of the cervix. Belongs to the insulin family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 9p24.1. Cellular Component: extracellular region. Molecular Function: hormone activity. Biological Process: female pregnancy; signal transduction

48-Strip-Wells

470 USD

96-Strip-Wells

675