ELISA/assay

Human Anterior Gradient Homolog 2 ELISA Kit

MBS723988

48-Strip-Wells-(Comp

470 USD

ELISA Kit

Human Anterior Gradient Homolog 2 ELISA Kit

Anterior Gradient Homolog 2

anterior gradient protein 2 homolog; Anterior gradient protein 2 homolog; anterior gradient protein 2 homolog; AG-2; HPC8; anterior gradient homolog 2; secreted cement gland homolog; epididymis secretory protein Li 116; secreted cement gland protein XAG-2 homolog; protein disulfide isomerase family A, member 17; anterior gradient 2; HPC8; Secreted cement gland protein XAG-2 homolog

AGR2

AGR2; AGR2; AG2; GOB-4; HAG-2; XAG-2; PDIA17; HEL-S-116; AG2; UNQ515/PRO1030; AG-2; hAG-2

AGR2_HUMAN

Human

Store all reagents at 2-8 degree C.

Assay Type: Competitive or Sandwich. Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate

Intended Uses: This AGR2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human AGR2. This ELISA kit for research use only, not for therapeutic or diagnostic applications. !Intended Uses: This AGR2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human AGR2. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Signal Transduction

Principle of the Assay: AGR2 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for AGR2. Standards or samples are then added to the microtiter plate wells and AGR2 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of AGR2 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for AGR2 are added to each well to "sandwich" the AGR2 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain AGR2 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AGR2 concentration in each sample is interpolated from this standard curve.

5453541

NP_006399.1

NM_006408.3

19,979 Da

AGR2: Required for MUC2 post-transcriptional synthesis and secretion. May play a role in the production of mucus by intestinal cells. Proto-oncogene that may play a role in cell migration, cell differentiation and cell growth. Interacts with LYPD3 and DAG1 (alphaDAG1). Interacts with MUC2; disulfide-linked. Expressed strongly in trachea, lung, stomach, colon, prostate and small intestine. Expressed weakly in pituitary gland, salivary gland, mammary gland, bladder, appendix, ovary, fetal lung, uterus, pancreas, kidney, fetal kidney, testis, placenta, thyroid gland and in estrogen receptor (ER)-positive breast cancer cell lines. Belongs to the AGR family. Protein type: Secreted; Oncoprotein; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 7p21.3. Cellular Component: mitochondrion; endoplasmic reticulum; extracellular region. Molecular Function: protein binding

48-Strip-Wells-(Competitive)

470 USD

48-Strip-Wells-(Sandwich)

470

96-Strip-Wells-(Competitive)

675

96-Strip-Wells-(Sandwich)

675