ELISA/assay

Mouse Acetyl-CoA carboxylase 2 (ACACB) ELISA Kit

MBS7239707

48-Strip-Wells

440 USD

ELISA Kit

Mouse Acetyl-CoA carboxylase 2 (ACACB) ELISA Kit

[Acetyl-CoA carboxylase 2 (ACACB)]

[acetyl-CoA carboxylase 2; Acetyl-CoA carboxylase 2; acetyl-CoA carboxylase 2; ACC-beta; acetyl-Coenzyme A carboxylase beta; acetyl-CoA carboxylase beta; ACC-betaIncluding the following 1 domains:Biotin carboxylase (EC:6.3.4.14)]

[ACACB]

[ACACB; ACACB; ACC2; ACCB; HACC275; ACC2; ACCB]

ACACB_HUMAN

Mouse

This assay has high sensitivity and excellent specificity for detection of ACAb-IgA. No significant cross-reactivity or interference between ACAb-IgA and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ACAb-IgA and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, Cell Culture Supernatants, Body Fluid And Tissue Homogenate. Assay Type: Quantitative Competitive. Sensitivity: 1.0 ng/mL.

Intended Uses: This ACAb-IgA ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat ACAb-IgA. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: ACAb-IgA ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-ACAb-IgA antibody and an ACAb-IgA-HRP conjugate. The assay sample and buffer are incubated together with ACAb-IgA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ACAb-IgA concentration since ACAb-IgA from samples and ACAb-IgA-HRP conjugate compete for the anti-ACAb-IgA antibody binding site. Since the number of sites is limited, as more sites are occupied by ACAb-IgA from the sample, fewer sites are left to bind ACAb-IgA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ACAb-IgA concentration in each sample is interpolated from this standard curve.

134142062

NP_001084.3

NM_001093.3

268,166 Da

AMPK Signaling Pathway (198868); AMPK Signaling Pathway (989139); AMPK Signaling Pathway (992181); Activation Of Gene Expression By SREBF (SREBP) Pathway (685552); Adipocytokine Signaling Pathway (83093); Adipocytokine Signaling Pathway (505); BDNF Signaling Pathway (712093); Biotin Transport And Metabolism Pathway (106257); ChREBP Activates Metabolic Gene Expression Pathway (106104); Defective AMN Causes Hereditary Megaloblastic Anemia 1 Pathway (906000)

Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system. ACC is a biotin-containing enzyme which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. ACC-beta is thought to control fatty acid oxidation by means of the ability of malonyl-CoA to inhibit carnitine-palmitoyl-CoA transferase I, the rate-limiting step in fatty acid uptake and oxidation by mitochondria. ACC-beta may be involved in the regulation of fatty acid oxidation, rather than fatty acid biosynthesis. There is evidence for the presence of two ACC-beta isoforms. [provided by RefSeq, Jul 2008]

ACC2: a subunit of acetyl-CoA carboxylase (ACC), a multifunctional enzyme system. Catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. Activity is increased by oligomerization. Activated by citrate. Citrate and MID1IP1 promote oligomerization. Inhibited by malonyl-CoA. ACC1 is the predominant form expressed in liver, adipocyte and mammary gland. Phosphorylation by AMPK or PKA inhibits the enzymatic activity of ACC. ACC2 is the major isoform in skeletal muscle and heart. Phosphorylation regulates its activity. Two isoforms of the human protein are produced by alternative splicing. Protein type: EC 6.3.4.14; Carbohydrate Metabolism - pyruvate; Ligase; Mitochondrial; EC 6.4.1.2; Carbohydrate Metabolism - propanoate; Lipid Metabolism - fatty acid biosynthesis. Chromosomal Location of Human Ortholog: 12q24.11. Cellular Component: mitochondrial outer membrane; mitochondrion; endomembrane system; cytosol; nucleus. Molecular Function: protein binding; acetyl-CoA carboxylase activity; metal ion binding; biotin carboxylase activity; biotin binding; ATP binding. Biological Process: response to drug; vitamin metabolic process; acetyl-CoA metabolic process; energy reserve metabolic process; positive regulation of cellular metabolic process; carnitine shuttle; cellular lipid metabolic process; response to organic cyclic substance; negative regulation of fatty acid oxidation; water-soluble vitamin metabolic process; fatty acid biosynthetic process; biotin metabolic process; protein homotetramerization

48-Strip-Wells

440 USD

96-Strip-Wells

640

5x96-Strip-Wells

2895

10x96-Strip-Wells

5415