product summary
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company name :
MyBioSource
product type :
ELISA/assay
product name :
Mouse alpha Melanocyte Stimulating Hormone ELISA Kit
catalog :
MBS723969
quantity :
48-Strip-Wells-(Comp
price :
470 USD
more info or order :
MyBioSource product webpage
citations: 1
Reference
Souza G, Solon C, Nascimento L, De Lima Junior J, Nogueira G, Moura R, et al. Defective regulation of POMC precedes hypothalamic inflammation in diet-induced obesity. Sci Rep. 2016;6:29290 pubmed publisher
product information
catalog number :
MBS723969
products type :
ELISA Kit
products full name :
Mouse alpha Melanocyte Stimulating Hormone ELISA Kit
products short name :
alpha Melanocyte Stimulating Hormone
products name syn :
Mouse a Melanocyte Stimulating Hormone ELISA Kit
other names :
alpha-melanocyte-stimulating hormone, partial; Alpha-melanocyte-stimulating hormone; Alpha-melanocyte-stimulating hormone
products gene name :
alpha-MSH
products gene name syn :
a-MSH
other gene names :
POMC
uniprot entry name :
D2JZU1_CAPHI
reactivity :
Mouse
specificity :
This assay has high sensitivity and excellent specificity for detection of alphaMSH. No significant cross-reactivity or interference between alphaMSH and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between alphaMSH and all the analogues, therefore, cross reaction may still exist in some cases.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Competitive or Sandwich. Sensitivity: 1.0 pg/mL.
products categories :
Signal Transduction
products description :
Intended Uses This alphaMSH ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse alphaMSH. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: alphaMSH ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-alphaMSH antibody and an alphaMSH-HRP conjugate. The assay sample and buffer are incubated together with alphaMSH-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the alphaMSH concentration since alphaMSH from samples and alphaMSH-HRP conjugate compete for the anti-alphaMSH antibody binding site. Since the number of sites is limited, as more sites are occupied by alphaMSH from the sample, fewer sites are left to bind alphaMSH-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The alphaMSH concentration in each sample is interpolated from this standard curve.
ncbi gi num :
269979223
ncbi acc num :
ACZ56127.1
ncbi mol weight :
5,602 Da
size1 :
48-Strip-Wells-(Competitive)
price1 :
470 USD
size2 :
48-Strip-Wells-(Sandwich)
price2 :
470
size3 :
96-Strip-Wells-(Competitive)
price3 :
675
size4 :
96-Strip-Wells-(Sandwich)
price4 :
675
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."

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