catalog number :
MBS723947
products type :
ELISA Kit
products full name :
Human Adipocyte Fatty Acid Binding Protein ELISA Kit
products short name :
Adipocyte Fatty Acid Binding Protein
other names :
A-FABP, partial; A-FABP; A-FABP
products gene name :
A FABP
uniprot entry name :
A9YWQ7_ANAPL
specificity :
This assay has high sensitivity and excellent specificity for detection of alphaLALB. No significant cross-reactivity or interference between alphaLALB and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between alphaLALB and all the analogues, therefore, cross reaction may still exist in some cases.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 pg/mL.
other info2 :
Intended Uses: This alphaLALB ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human alphaLALB. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
products categories :
Cardiovascular
products description :
Principle of the assay: alphaLALB ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-alphaLALB antibody and an alphaLALB-HRP conjugate. The assay sample and buffer are incubated together with alphaLALB-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the alphaLALB concentration since alphaLALB from samples and alphaLALB-HRP conjugate compete for the anti-alphaLALB antibody binding site. Since the number of sites is limited, as more sites are occupied by alphaLALB from the sample, fewer sites are left to bind alphaLALB-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The alphaLALB concentration in each sample is interpolated from this standard curve.
ncbi acc num :
ABY27083.1
ncbi mol weight :
3,816 Da