ELISA/assay

Human Involucrin ELISA Kit

MBS723946

48-Strip-Wells-(Comp

470 USD

ELISA Kit

Human Involucrin ELISA Kit

Involucrin

involucrin; Involucrin; involucrin; involucrin

INV

IVL; IVL

INVO_HUMAN

Human

Store all reagents at 2-8 degree C.

Assay Type: Competitive or Sandwich. Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Sensitivity: 0.1 ng/mL.

Signal Transduction

Intended Uses: This IL ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human IL. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: IL ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-IL antibody and an IL-HRP conjugate. The assay sample and buffer are incubated together with IL-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IL concentration since IL from samples and IL-HRP conjugate compete for the anti-IL antibody binding site. Since the number of sites is limited, as more sites are occupied by IL from the sample, fewer sites are left to bind IL-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL concentration in each sample is interpolated from this standard curve.

44890059

NP_005538.2

NM_005547.2

68,479 Da

Corticotropin-releasing Hormone Pathway (920957); Validated Transcriptional Targets Of AP1 Family Members Fra1 And Fra2 Pathway (169349)

Involucrin, a component of the keratinocyte crosslinked envelope, is found in the cytoplasm and crosslinked to membrane proteins by transglutaminase. This gene is mapped to 1q21, among calpactin I light chain, trichohyalin, profillaggrin, loricrin, and calcyclin. [provided by RefSeq, Jul 2008]

IVL: Part of the insoluble cornified cell envelope (CE) of stratified squamous epithelia. Belongs to the involucrin family. Chromosomal Location of Human Ortholog: 1q21. Cellular Component: cytoplasm; cornified envelope. Molecular Function: protein binding, bridging; structural molecule activity. Biological Process: keratinocyte differentiation; isopeptide cross-linking via N6-(L-isoglutamyl)-L-lysine; keratinization; peptide cross-linking; response to UV-B

48-Strip-Wells-(Competitive)

470 USD

48-Strip-Wells-(Sandwich)

470

96-Strip-Wells-(Competitive)

675

96-Strip-Wells-(Sandwich)

675