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company name :
MyBioSource
product type :
ELISA/assay
product name :
Mouse Anti spermiogenesis Antibody ELISA Kit
catalog :
MBS723930
quantity :
48-Strip-Wells
price :
470 USD
more info or order :
product information
catalog number :
MBS723930
products type :
ELISA Kit
products full name :
Mouse Anti spermiogenesis Antibody ELISA Kit
products short name :
Anti spermiogenesis Antibody
products gene name :
ASAb
reactivity :
Mouse
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Cell culture fluid & body fluid & tissue homogenate Serum or blood plasma
products categories :
Immunology
products description :
For Samples: Cell culture fluid & body fluid & tissue homogenate serum or blood plasma. Intended Uses: This ASAb ELISA kit is intended for laboratory research use only and not for use in diagnostic or therapeutic procedures. The stop solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of ASAb in the sample, this ASAb ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus ASAb concentration. The concentration of in the samples is then determined by comparing the O.D. of the samples to the standard curve. Principle of the Assay: The coated well immunoenzymatic assay for the quantitative measurement of ASAb utilizes a polyclonal anti-ASAb antibody and an ASAb-HRP conjugate. The assay sample and buffer are incubated together with ASAb-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ASAb concentration since ASAb from samples and ASAb-HRP conjugate compete for the anti-ASAb antibody binding site. Since the number of sites is limited, as more sites are occupied by ASAb from the sample, fewer sites are left to bind ASAb-HRP conjugate. Standards of known ASAb concentrations are run concurrently with the samples being assayed and a standard curve is plotted relating the intensity of the color (O.D.) to the concentration of ASAb. The ASAb concentration in each sample is interpolated from this standard curve.
size1 :
48-Strip-Wells
price1 :
470 USD
size2 :
96-Strip-Wells
price2 :
675
more info or order :
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.
"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."
Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...
"MyBioSource offers the best products at unbeatable prices."
Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.
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