ELISA/assay

Rat Complement factor H-related protein 2 (CFHR2) ELISA Kit

MBS7239247

48-Strip-Wells

470 USD

ELISA Kit

Rat Complement factor H-related protein 2 (CFHR2) ELISA Kit

Complement factor H-related protein 2 (CFHR2)

complement factor H-related protein 2; Complement factor H-related protein 2; complement factor H-related protein 2; FHR-2; DDESK59; h factor-like 3; factor H-related gene 2; h factor-like protein 2; H factor (complement)-like 3; complement factor H-related 2; DDESK59; H factor-like 3; H factor-like protein 2

CFHR2

CFHR2; CFHR2; FHR2; HFL3; CFHL2; CFHL2; FHR2; HFL3; FHR-2

FHR2_HUMAN

Rat

This assay has high sensitivity and excellent specificity for detection of CFHR2. No significant cross-reactivity or interference between CFHR2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CFHR2 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 ng/mL.

Intended Uses: This CFHR2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat CFHR2. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Immunology

Principle of the assay: CFHR2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CFHR2 antibody and an CFHR2-HRP conjugate. The assay sample and buffer are incubated together with CFHR2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CFHR2 concentration since CFHR2 from samples and CFHR2-HRP conjugate compete for the anti-CFHR2 antibody binding site. Since the number of sites is limited, as more sites are occupied by CFHR2 from the sample, fewer sites are left to bind CFHR2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CFHR2 concentration in each sample is interpolated from this standard curve.

5031695

NP_005657.1

NM_005666.2

27,897 Da

This gene belongs to a family of complement factor H-related genes (CFHR), which are clustered together with complement factor H gene on chromosome 1, and are involved in regulation of complement. Mutations in CFHR genes have been associated with dense deposit disease and atypical haemolytic-uraemic syndrome. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Aug 2015]

CFHR2: Might be involved in complement regulation. Can associate with lipoproteins and may play a role in lipid metabolism. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 1q31.3. Cellular Component: extracellular region

48-Strip-Wells

470 USD

96-Strip-Wells

675