Mouse N-acetyllactosaminide alpha-1, 3-galactosyltransferase (GGTA1) ELISA Kit | MyBioSource MBS7239150 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse N-acetyllactosaminide alpha-1, 3-galactosyltransferase (GGTA1) ELISA Kit

catalog :

MBS7239150

quantity :

48-Strip-Wells

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS7239150

products type :

ELISA Kit

products full name :

Mouse N-acetyllactosaminide alpha-1, 3-galactosyltransferase (GGTA1) ELISA Kit

products short name :

N-acetyllactosaminide alpha-1, 3-galactosyltransferase (GGTA1)

other names :

N-acetyllactosaminide alpha-1,3-galactosyltransferase isoform 2; N-acetyllactosaminide alpha-1,3-galactosyltransferase; N-acetyllactosaminide alpha-1,3-galactosyltransferase; alpha Gal; glycoprotein alpha galactosyl transferase 1; UDP-galactose:beta-D-galactosyl-1,4-N-acetyl-D-glucosaminide alpha-1,3-galactosyltransferase; glycoprotein galactosyltransferase alpha 1, 3; UDP-galactose:beta-D-galactosyl-1,4-N-acetyl-D-glucosaminide alpha-1,3-galactosyltransferase; Galactosyltransferase

products gene name :

GGTA1

other gene names :

Ggta1; Ggta1; Gal; GALT; Ggta; Ggta-1; AW108479; alpha3GalT; Ggta-1; Galactosyltransferase

uniprot entry name :

GGTA1_MOUSE

reactivity :

Mouse

specificity :

This assay has high sensitivity and excellent specificity for detection of GGTA1. No significant cross-reactivity or interference between GGTA1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between GGTA1 and all the analogues, therefore, cross reaction may still exist in some cases.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 ng/mL.

other info2 :

Intended Uses: This GGTA1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse GGTA1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

products description :

Principle of the assay: GGTA1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-GGTA1 antibody and an GGTA1-HRP conjugate. The assay sample and buffer are incubated together with GGTA1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GGTA1 concentration since GGTA1 from samples and GGTA1-HRP conjugate compete for the anti-GGTA1 antibody binding site. Since the number of sites is limited, as more sites are occupied by GGTA1 from the sample, fewer sites are left to bind GGTA1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GGTA1 concentration in each sample is interpolated from this standard curve.

ncbi gi num :

224922810

ncbi acc num :

NP_001139293.1

ncbi gb acc num :

NM_001145821.1

ncbi mol weight :

43,962 Da

ncbi pathways :

Glycosphingolipid Biosynthesis - Lacto And Neolacto Series Pathway (83194); Glycosphingolipid Biosynthesis - Lacto And Neolacto Series Pathway (370)

ncbi summary :

This gene encodes a member of the galactosyltransferase family of intracellular, membrane-bound enzymes that are involved in the biosynthesis of glycoproteins and glycolipids. The encoded protein catalyzes the transfer of galactose from UDP-galactose to N-acetyllactosamine in an alpha(1,3) linkage to form galactose alpha(1,3)-galactose. Mice lacking the encoded protein develop cortical cataracts. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, May 2015]

uniprot summary :

Ggta1: Transfer of galactose from UDP-galactose to an acceptor molecule (R). Belongs to the glycosyltransferase 6 family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral; EC 2.4.1.87. Cellular Component: Golgi apparatus; Golgi cisterna; membrane; integral to membrane. Molecular Function: transferase activity; transferase activity, transferring glycosyl groups; transferase activity, transferring hexosyl groups; metal ion binding; N-acetyllactosaminide 3-alpha-galactosyltransferase activity. Biological Process: protein amino acid galactosylation at cell surface; carbohydrate metabolic process

size1 :

48-Strip-Wells

price1 :

470 USD

size2 :

96-Strip-Wells

price2 :

675

more info or order :

MyBioSource product webpage