ELISA/assay

Bovine Hyaluronidase-1 (HYAL1) ELISA Kit

MBS7238849

48-Strip-Wells

470 USD

ELISA Kit

Bovine Hyaluronidase-1 (HYAL1) ELISA Kit

Hyaluronidase-1 (HYAL1)

hyaluronidase-1 isoform 2; Hyaluronidase-1; hyaluronidase-1; luCa-1; plasma hyaluronidase; tumor suppressor LUCA-1; lung carcinoma protein 1; hyaluronoglucosaminidase 1; Hyaluronoglucosaminidase-1; Lung carcinoma protein 1; LuCa-1

HYAL1

HYAL1; HYAL1; NAT6; LUCA1; HYAL-1; LUCA1; Hyal-1; LuCa-1

HYAL1_HUMAN

Bovine

This assay has high sensitivity and excellent specificity for detection of HYAL1. No significant cross-reactivity or interference between HYAL1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between HYAL1 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1ng/mL.

Intended Uses: This HYAL1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine HYAL1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Signal Transduction

Principle of the assay: HYAL1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-HYAL1 antibody and an HYAL1-HRP conjugate. The assay sample and buffer are incubated together with HYAL1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HYAL1 concentration since HYAL1 from samples and HYAL1-HRP conjugate compete for the anti-HYAL1 antibody binding site. Since the number of sites is limited, as more sites are occupied by HYAL1 from the sample, fewer sites are left to bind HYAL1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HYAL1 concentration in each sample is interpolated from this standard curve.

24497566

NP_695014.1

NM_153282.2

37,439 Da

CS/DS Degradation Pathway (645311); Chondroitin Sulfate Degradation Pathway (413376); Chondroitin Sulfate Degradation Pathway (468247); Chondroitin Sulfate/dermatan Sulfate Metabolism Pathway (645308); Dermatan Sulfate Degradation Pathway (413375); Dermatan Sulfate Degradation Pathway (468240); Disease Pathway (530764); Glycogen Storage Diseases Pathway (980468); Glycosaminoglycan Degradation Pathway (82981); Glycosaminoglycan Degradation Pathway (355)

This gene encodes a lysosomal hyaluronidase. Hyaluronidases intracellularly degrade hyaluronan, one of the major glycosaminoglycans of the extracellular matrix. Hyaluronan is thought to be involved in cell proliferation, migration and differentiation. This enzyme is active at an acidic pH and is the major hyaluronidase in plasma. Mutations in this gene are associated with mucopolysaccharidosis type IX, or hyaluronidase deficiency. The gene is one of several related genes in a region of chromosome 3p21.3 associated with tumor suppression. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

HYAL1: May have a role in promoting tumor progression. May block the TGFB1-enhanced cell growth. Defects in HYAL1 are the cause of mucopolysaccharidosis type 9 (MPS9); also called hyaluronidase deficiency. MPS9 is a lysosomal storage disease characterized by high hyaluronan (HA) concentration in the serum. The clinical features are periarticular soft tissue masses, mild short stature and acetabular erosions, and absence of neurological or visceral involvement. Belongs to the glycosyl hydrolase 56 family. 7 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted, signal peptide; EC 3.2.1.35; Glycan Metabolism - glycosaminoglycan degradation; Secreted; Hydrolase. Chromosomal Location of Human Ortholog: 3p21.31. Cellular Component: lysosomal lumen; extracellular space; lysosome; cytoplasm; cytoplasmic vesicle. Molecular Function: viral receptor activity; hyaluronan synthase activity; transcription factor binding; hyalurononglucosaminidase activity. Biological Process: positive regulation of cell adhesion; glycosaminoglycan metabolic process; response to virus; pathogenesis; positive regulation of cell growth; hyaluronan catabolic process; response to antibiotic; chondroitin sulfate metabolic process; positive regulation of angiogenesis; response to reactive oxygen species; hyaluronan biosynthetic process; cartilage development; carbohydrate metabolic process; chondroitin sulfate catabolic process; negative regulation of cell growth; inflammatory response; positive regulation of growth; hyaluronan metabolic process; positive regulation of epithelial cell proliferation. Disease: Mucopolysaccharidosis, Type Ix

48-Strip-Wells

470 USD

96-Strip-Wells

675