catalog number :
MBS723876
products type :
ELISA Kit
products full name :
Human Advanced Glycosylation End Products ELISA Kit
products short name :
Advanced Glycosylation End Products
products gene name :
AGEs
specificity :
This assay has high sensitivity and excellent specificity for detection of AGEs. No significant cross-reactivity or interference between AGEs and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between AGEs and all the analogues, therefore, cross reaction may still exist in some cases.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Sandwich. Sensitivity: 1.0pg/mL.
products description :
Intended Uses This AGEs ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human AGEs. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: AGEs ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for AGEs. Standards or samples are then added to the microtiter plate wells and AGEs if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of AGEs present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for AGEs are added to each well to "sandwichâ the AGEs immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain AGEs and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AGEs concentration in each sample is interpolated from this standard curve.