Human 14 3 3 protein gamma (YWHAG) ELISA Kit | MyBioSource MBS7238731 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human 14 3 3 protein gamma (YWHAG) ELISA Kit

catalog :

MBS7238731

quantity :

48-Strip-Wells

price :

440 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS7238731

products type :

ELISA Kit

products full name :

Human 14 3 3 protein gamma (YWHAG) ELISA Kit

products short name :

[14 3 3 protein gamma (YWHAG)]

other names :

[14-3-3 protein gamma; 14-3-3 protein gamma; 14-3-3 protein gamma; KCIP-1; 14-3-3 gamma; protein kinase C inhibitor protein 1; protein phosphatase 1, regulatory subunit 170; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma; Protein kinase C inhibitor protein 1; KCIP-1]

other gene names :

[YWHAG; YWHAG; PPP1R170; 14-3-3GAMMA; KCIP-1]

uniprot entry name :

1433G_HUMAN

reactivity :

Human

specificity :

This assay has high sensitivity and excellent specificity for detection of YWHAG. No significant cross-reactivity or interference between YWHAG and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between YWHAG and all the analogues, therefore, cross reaction may still exist in some cases.

storage stability :

Store all reagents at 2-8 degree C.

image1 heading :

Typical Standard Curve/Testing Data

other info1 :

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Detection Range: 50-1000ng/mL. Sensitivity: 1.0ng/mL

products categories :

Signal Transduction

products description :

Intended Uses: This YWHAG ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human YWHAG. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: YWHAG ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-YWHAG antibody and an YWHAG-HRP conjugate. The assay sample and buffer are incubated together with YWHAG-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the YWHAG concentration since YWHAG from samples and YWHAG-HRP conjugate compete for the anti-YWHAG antibody binding site. Since the number of sites is limited, as more sites are occupied by YWHAG from the sample, fewer sites are left to bind YWHAG-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The YWHAG concentration in each sample is interpolated from this standard curve.

ncbi gi num :

21464101

ncbi acc num :

NP_036611.2

ncbi gb acc num :

NM_012479.3

ncbi mol weight :

28,303 Da

ncbi pathways :

Activation Of BAD And Translocation To Mitochondria Pathway (105659); Activation Of BH3-only Proteins Pathway (105658); Apoptosis Pathway (105648); Calcium Regulation In The Cardiac Cell Pathway (198906); Cell Cycle Pathway (530733); Cell Cycle, Mitotic Pathway (105765); Cell Cycle Pathway (198811); Cell Cycle Pathway (83054); Cell Cycle Pathway (463); Centrosome Maturation Pathway (105807)

ncbi summary :

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the rat ortholog. It is induced by growth factors in human vascular smooth muscle cells, and is also highly expressed in skeletal and heart muscles, suggesting an important role for this protein in muscle tissue. It has been shown to interact with RAF1 and protein kinase C, proteins involved in various signal transduction pathways. [provided by RefSeq, Jul 2008]

uniprot summary :

14-3-3 gamma: Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Homodimer. Interacts with SAMSN1. Interacts with RAF1, SSH1 and CRTC2/TORC2. Interacts with ABL1 (phosphorylated form); the interaction retains it in the cytoplasm. Interacts with GAB2. Interacts with MDM4 (phosphorylated); negatively regulates MDM4 activity toward TP53. Interacts with PKA-phosphorylated AANAT. Highly expressed in brain, skeletal muscle, and heart. Belongs to the 14-3-3 family. Protein type: Adaptor/scaffold. Chromosomal Location of Human Ortholog: 7q11.23. Cellular Component: cytoplasmic vesicle membrane; focal adhesion; membrane; cytosol. Molecular Function: insulin-like growth factor receptor binding; protein domain specific binding; protein kinase C inhibitor activity; protein binding; protein kinase C binding; receptor tyrosine kinase binding. Biological Process: cellular response to insulin stimulus; apoptosis; regulation of neuron differentiation; organelle organization and biogenesis; regulation of signal transduction; regulation of synaptic plasticity; negative regulation of protein kinase activity; mitotic cell cycle; G2/M transition of mitotic cell cycle; protein targeting

size1 :

48-Strip-Wells

price1 :

440 USD

size2 :

96-Strip-Wells

price2 :

640

size3 :

5x96-Strip-Wells

price3 :

2895

size4 :

10x96-Strip-Wells

price4 :

5415

more info or order :

MyBioSource product webpage