ELISA/assay

Human Glutamate receptor 4 (GRIA4) ELISA Kit

MBS7238648

48-Strip-Wells

440 USD

ELISA Kit

Human Glutamate receptor 4 (GRIA4) ELISA Kit

[Glutamate receptor 4 (GRIA4)]

[glutamate receptor 4 isoform 1; Glutamate receptor 4; glutamate receptor 4; gluR-4; gluR-D; AMPA-selective glutamate receptor 4; glutamate receptor, ionotrophic, AMPA 4; glutamate receptor, ionotropic, AMPA 4; AMPA-selective glutamate receptor 4; GluR-D; Glutamate receptor ionotropic, AMPA 4; GluA4]

[GRIA4; GRIA4; GLUR4; GLURD; GluA4; GLUR4C; GLUR4; GluR-4; GluR4; GluA4]

GRIA4_HUMAN

Human

This assay has high sensitivity and excellent specificity for detection of GRIA4. No significant cross-reactivity or interference between GRIA4 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between GRIA4 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Detection Range: 2.5-50ng/mL. Sensitivity: 0.1ng/mL

Neurobiology

Intended Uses: This GRIA4 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human GRIA4. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay GRIA4 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-GRIA4 antibody and an GRIA4-HRP conjugate. The assay sample and buffer are incubated together with GRIA4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GRIA4 concentration since GRIA4 from samples and GRIA4-HRP conjugate compete for the anti-GRIA4 antibody binding site. Since the number of sites is limited, as more sites are occupied by GRIA4 from the sample, fewer sites are left to bind GRIA4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GRIA4 concentration in each sample is interpolated from this standard curve.

164419734

NP_000820.3

NM_000829.3

49,146 Da

Activation Of AMPA Receptors Pathway (106539); Activation Of NMDA Receptor Upon Glutamate Binding And Postsynaptic Events Pathway (161033); Amphetamine Addiction Pathway (547607); Amphetamine Addiction Pathway (550546); Circadian Entrainment Pathway (698773); Circadian Entrainment Pathway (699872); Dopaminergic Synapse Pathway (469199); Dopaminergic Synapse Pathway (469185); Glutamate Binding, Activation Of AMPA Receptors And Synaptic Plasticity Pathway (106536); Glutamatergic Synapse Pathway (213818)

Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. These receptors are heteromeric protein complexes composed of multiple subunits, arranged to form ligand-gated ion channels. The classification of glutamate receptors is based on their activation by different pharmacologic agonists. The subunit encoded by this gene belongs to a family of AMPA (alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate)-sensitive glutamate receptors, and is subject to RNA editing (AGA- R- G). Alternative splicing of this gene results in transcript variants encoding different isoforms, which may vary in their signal transduction properties. Some haplotypes of this gene show a positive association with schizophrenia. [provided by RefSeq, Jul 2008]

GluR4: an integral membrane protein belonging to the glutamate-gated ion channel family. L-glutamate (Glu) acts as an excitatory neurotransmitter at many synapses in the central nervous system. Glutamate receptors are heteromeric protein complexes with multiple subunits, each possessing transmembrane regions, and all arranged to form a ligand-gated ion channel. The postsynaptic actions of Glu are mediated by a variety of receptors that are named according to their selective agonists. This receptor binds AMPA(quisqualate) glutamate kainate. Each of the four GluR proteins (GRIA1-4) include flip and flop isoforms generated by alternative RNA splicing. Protein type: Channel, ligand-gated; Membrane protein, multi-pass; Membrane protein, integral. Chromosomal Location of Human Ortholog: 11q22. Cellular Component: postsynaptic membrane; dendrite; plasma membrane; cell junction; vesicle. Molecular Function: extracellular-glutamate-gated ion channel activity; ionotropic glutamate receptor activity; alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate selective glutamate receptor activity. Biological Process: synaptic transmission, glutamatergic; synaptic transmission; glutamate signaling pathway; transport; ionotropic glutamate receptor signaling pathway

48-Strip-Wells

440 USD

96-Strip-Wells

640

5x96-Strip-Wells

2895

10x96-Strip-Wells

5415