ELISA/assay

Rat Corticotropin Releasing Factor ELISA Kit

MBS723862

48-Strip-Wells

440 USD

ELISA Kit

Rat Corticotropin Releasing Factor ELISA Kit

[Corticotropin Releasing Factor]

[corticoliberin preproprotein; Corticoliberin; corticoliberin; corticotropin-releasing factor; corticotropin releasing hormone; Corticotropin-releasing factor; CRF; Corticotropin-releasing hormone]

[CRF]

[CRH; CRH; CRF; CRF]

CRF_HUMAN

Rat

This assay has high sensitivity and excellent specificity for detection of CREM. No significant cross-reactivity or interference between CREM and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CREM and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, Cell Culture Supernatants, Body Fluid And Tissue Homogenate. Assay Type: Quantitative Competitive. Sensitivity: 0.1 ng/mL.

Neurobiology

Intended Uses: This CREM ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of HumanCREM. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: CREM ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CREM antibody and an CREM-HRP conjugate. The assay sample and buffer are incubated together with CREM-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CREM concentration since CREM from samples and CREM-HRP conjugate compete for the anti-CREM antibody binding site. Since the number of sites is limited, as more sites are occupied by CREM from the sample, fewer sites are left to bind CREM-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CREM concentration in each sample is interpolated from this standard curve.

4503041

NP_000747.1

NM_000756.2

21,422 Da

Alcoholism Pathway (585563); Alcoholism Pathway (587116); Class B/2 (Secretin Family Receptors) Pathway (106378); Corticotropin-releasing Hormone Pathway (920957); G Alpha (s) Signalling Events Pathway (119549); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020); Long-term Depression Pathway (83086); Long-term Depression Pathway (497); Myometrial Relaxation And Contraction Pathways (198759)

Corticotropin-releasing hormone is secreted by the paraventricular nucleus (PVN) of the hypothalamus in response to stress. Marked reduction in this protein has been observed in association with Alzheimer disease and autosomal recessive hypothalamic corticotropin deficiency has multiple and potentially fatal metabolic consequences including hypoglycemia and hepatitis. In addition to production in the hypothalamus, this protein is also synthesized in peripheral tissues, such as T lymphocytes and is highly expressed in the placenta. In the placenta it is a marker that determines the length of gestation and the timing of parturition and delivery. A rapid increase in circulating levels of the hormone occurs at the onset of parturition, suggesting that, in addition to its metabolic functions, this protein may act as a trigger for parturition. [provided by RefSeq, Apr 2010]

CRH: This hormone from hypothalamus regulates the release of corticotropin from pituitary gland. Belongs to the sauvagine/corticotropin-releasing factor/urotensin I family. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 8q13. Cellular Component: extracellular space; cytoplasm; extracellular region; perikaryon. Molecular Function: corticotropin-releasing hormone receptor 1 binding; neuropeptide hormone activity; protein binding; hormone activity; corticotropin-releasing hormone receptor 2 binding; receptor binding; adrenocorticotropin-releasing hormone activity. Biological Process: negative regulation of circadian sleep/wake cycle, REM sleep; diterpenoid metabolic process; synaptic transmission, dopaminergic; hypothalamus development; positive regulation of cortisol secretion; induction of apoptosis by hormones; response to pain; female pregnancy; signal transduction; positive regulation of adrenocorticotropic hormone secretion; negative regulation of luteinizing hormone secretion; synaptic transmission; learning and/or memory; positive regulation of cell proliferation; positive regulation of cAMP biosynthetic process; feeding behavior; parturition; negative regulation of blood pressure; inflammatory response; response to electrical stimulus; associative learning; response to corticosterone stimulus; response to drug; adrenal gland development; response to ether; response to ethanol; long-term memory; response to estrogen stimulus; ion homeostasis; regulation of serotonin secretion; negative regulation of epinephrine secretion; positive regulation of protein amino acid phosphorylation; glucocorticoid biosynthetic process; lung development. Disease: Acth Deficiency, Isolated

48-Strip-Wells

440 USD

96-Strip-Wells

640

5x96-Strip-Wells

2895

10x96-Strip-Wells

5415