catalog number :
MBS723854
products type :
ELISA Kit
products full name :
Rat N-acetyl-beta-D-glucosaminidase ELISA Kit
products short name :
[N-acetyl-beta-D-glucosaminidase]
products name syn :
[Rat N-acetyl-b-D-glucosaminidase ELISA Kit]
other names :
[NAG, partial; Bifunctional autolysin]
products gene name :
[NAG]
products gene name syn :
[NAG]
other gene names :
[atl; nag]
uniprot entry name :
ATL_STAAU
specificity :
This assay has high sensitivity and excellent specificity for detection of NADR. No significant cross-reactivity or interference between NADR and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between NADR and all the analogues, therefore, cross reaction may still exist in some cases.
storage stability :
Store all reagents at 2-8 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma, Cell Culture Supernatants, Body Fluid And Tissue Homogenate. Assay Type: Quantitative Competitive. Sensitivity: 1.0 ng/mL.
products description :
Intended Uses: This NADR ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human NADR. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: NADR ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-NADR antibody and an NADR-HRP conjugate. The assay sample and buffer are incubated together with NADR-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the NADR concentration since NADR from samples and NADR-HRP conjugate compete for the anti-NADR antibody binding site. Since the number of sites is limited, as more sites are occupied by NADR from the sample, fewer sites are left to bind NADR-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NADR concentration in each sample is interpolated from this standard curve.
ncbi acc num :
BAA22600.1
ncbi mol weight :
137,535 Da
size1 :
48-Strip-Wells-(Competitive)
size2 :
48-Strip-Wells-(Sandwich)
size3 :
96-Strip-Wells-(Competitive)
size4 :
96-Strip-Wells-(Sandwich)
size5 :
5x96-Strip-Wells-(Competitive)
size6 :
5x96-Strip-Wells-(Sandwich)
size7 :
10x96-Strip-Wells-(Competitive)
size8 :
10x96-Strip-Wells-(Sandwich)