catalog number :
MBS7238484
products type :
ELISA Kit
products full name :
Human Insulin like protein 3 (INSL3) ELISA Kit
products short name :
[Insulin like protein 3 (INSL3)]
products gene name :
[INSL3]
specificity :
This assay has high sensitivity and excellent specificity for detection of INSL3. No significant cross-reactivity or interference between INSL3 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between INSL3 and all the analogues, therefore, cross reaction may still exist in some cases.
storage stability :
Store all reagents at 2-8 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Detection Range: 50-1000ng/mL. Sensitivity: 1.0ng/mL
products categories :
Signal Transduction
products description :
Intended Uses: This INSL3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human INSL3. Principle of the Assay: INSL3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-INSL3 antibody and an INSL3-HRP conjugate. The assay sample and buffer are incubated together with INSL3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the INSL3 concentration since INSL3 from samples and INSL3-HRP conjugate compete for the anti-INSL3 antibody binding site. Since the number of sites is limited, as more sites are occupied by INSL3 from the sample, fewer sites are left to bind INSL3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The INSL3 concentration in each sample is interpolated from this standard curve.
size4 :
10x96-Strip-Wells