ELISA/assay

Mouse Gamma-aminobutyric acid ELISA Kit

MBS723819

48-Strip-Wells

470 USD

ELISA Kit

Mouse Gamma-aminobutyric acid ELISA Kit

Gamma-aminobutyric acid

Mouse Gamma-aminobutyric acid ELISA Kit; Gamma-aminobutyric acid; Gamma-aminobutyric acid (Mouse)

Gaba; Gaba

GABA

Bm1_46715

Mouse

This assay has high sensitivity and excellent specificity for detection of gamma-ABA. No significant cross-reactivity or interference between gamma-ABA and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between gamma-ABA and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 pg/mL.

Intended Uses: This gamma-ABA ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse gamma-ABA. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Mouse ELISA Kit

Principle of the assay: gamma-ABA ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-gamma-ABA antibody and an gamma-ABA-HRP conjugate. The assay sample and buffer are incubated together with gamma-ABA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the gamma-ABA concentration since gamma-ABA from samples and gamma-ABA-HRP conjugate compete for the anti-gamma-ABA antibody binding site. Since the number of sites is limited, as more sites are occupied by gamma-ABA from the sample, fewer sites are left to bind gamma-ABA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The gamma-ABA concentration in each sample is interpolated from this standard curve.

170592096

XP_001900805.1

Regulation Of Autophagy Pathway (89224); Regulation Of Autophagy Pathway (468)

48-Strip-Wells

470 USD

96-Strip-Wells

675