ELISA/assay

Chicken Caspase recruitment domain-containing protein 11 (CARD11) ELISA Kit

MBS7238059

48-Strip-Wells

470 USD

ELISA Kit

Chicken Caspase recruitment domain-containing protein 11 (CARD11) ELISA Kit

Caspase recruitment domain-containing protein 11 (CARD11)

caspase recruitment domain-containing protein 11; Caspase recruitment domain-containing protein 11; caspase recruitment domain-containing protein 11; carma 1; CARD-containing MAGUK protein 1; bcl10-interacting maguk protein 3; caspase recruitment domain family, member 11; CARD-containing MAGUK protein 1; Carma 1

CARD11

CARD11; CARD11; PPBL; BENTA; BIMP3; IMD11; CARMA1; CARMA1; Carma 1

CAR11_HUMAN

Chicken

This assay has high sensitivity and excellent specificity for detection of CARD-11. No significant cross-reactivity or interference between CARD-11 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CARD-11 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Intended Uses: This CARD-11 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Chicken CARD-11. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the assay: CARD-11 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CARD-11 antibody and an CARD-11-HRP conjugate. The assay sample and buffer are incubated together with CARD-11-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CARD-11 concentration since CARD-11 from samples and CARD-11-HRP conjugate compete for the anti-CARD-11 antibody binding site. Since the number of sites is limited, as more sites are occupied by CARD-11 from the sample, fewer sites are left to bind CARD-11-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CARD-11 concentration in each sample is interpolated from this standard curve.

157743265

NP_115791.3

NM_032415.5

133,284 Da

Activation Of NF-kappaB In B Cells Pathway (576251); Adaptive Immune System Pathway (366160); B Cell Receptor Signaling Pathway (198909); B Cell Receptor Signaling Pathway (83081); B Cell Receptor Signaling Pathway (492); BCR Signaling Pathway (138058); Downstream TCR Signaling Pathway (106418); Downstream Signaling Events Of B Cell Receptor (BCR) Pathway (576250); FCERI Mediated NF-kB Activation Pathway (833828); Fc Epsilon Receptor (FCERI) Signaling Pathway (833825)

The protein encoded by this gene belongs to the membrane-associated guanylate kinase (MAGUK) family, a class of proteins that functions as molecular scaffolds for the assembly of multiprotein complexes at specialized regions of the plasma membrane. This protein is also a member of the CARD protein family, which is defined by carrying a characteristic caspase-associated recruitment domain (CARD). This protein has a domain structure similar to that of CARD14 protein. The CARD domains of both proteins have been shown to specifically interact with BCL10, a protein known to function as a positive regulator of cell apoptosis and NF-kappaB activation. When expressed in cells, this protein activated NF-kappaB and induced the phosphorylation of BCL10. [provided by RefSeq, Jul 2008]

CARD11: a CARD (caspase recruitment domain) protein that belongs to the membrane-associated guanylate kinase (MAGUK) family. MAGUK proteins function as molecular scaffolds for the assembly of multiprotein complexes at specialized regions of the plasma membrane. Contains an N-terminal CARD domain, a central coiled-coil domain, and a C-terminal region containing a PDZ, an SH3, and a GUK. Its CARD domain associates with the CARD domain of BCL10. BCL10 activates NF-kappaB through the IkappaB kinase complex in response to upstream stimuli. Apparently activates NF-kappaB and induce the phosphorylation of BCL10. Detected in adult peripheral blood leukocytes, thymus, spleen and liver. Also found in promyelocytic leukemia HL-60 cells, chronic myelogenous leukemia K562 cells, Burkitt s lymphoma Raji cells and colorectal adenocarcinoma SW480 cells. Protein type: Adaptor/scaffold. Chromosomal Location of Human Ortholog: 7p22. Cellular Component: T cell receptor complex; cytoplasm; plasma membrane; immunological synapse; cytosol; lipid raft. Molecular Function: guanylate kinase activity; protein binding; CARD domain binding. Biological Process: positive regulation of cytokine production; thymic T cell selection; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of interleukin-2 biosynthetic process; T cell receptor signaling pathway; activation of NF-kappaB transcription factor; regulation of apoptosis; regulation of B cell differentiation; T cell costimulation; positive regulation of B cell proliferation; innate immune response; regulation of T cell differentiation; positive regulation of T cell proliferation; nucleotide phosphorylation. Disease: Persistent Polyclonal B-cell Lymphocytosis; Immunodeficiency 11; B-cell Expansion With Nfkb And T-cell Anergy

48-Strip-Wells

470 USD

96-Strip-Wells

675