ELISA/assay

Human Ceruloplasmin (CP) ELISA Kit

MBS723792

48-Strip-Wells

470 USD

ELISA Kit

Human Ceruloplasmin (CP) ELISA Kit

Ceruloplasmin (CP)

Human Ceruloplasmin (CP) ELISA Kit; Ceruloplasmin (CP); Ceruloplasmin ELISA Kit (CP) (Human)

ceruloplasmin; Ceruloplasmin; ceruloplasmin; OTTHUMP00000197026; OTTHUMP00000215840; OTTHUMP00000215841; ceruloplasmin (ferroxidase); Ferroxidase

CP

CP; CP; CP-2

CERU_HUMAN

Human

This assay has high sensitivity and excellent specificity for detection of CP. No significant cross-reactivity or interference between CP and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CP and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Sandwich. Sensitivity: 1.0 mug/dl.

Intended Uses: This CP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CP. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Human ELISA Kit

Principle of the assay: CP ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for CP. Standards or samples are then added to the microtiter plate wells and CP if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of CP present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for CP are added to each well to "sandwich" the CP immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain CP and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CP concentration in each sample is interpolated from this standard curve.

1731685

P00450

122,205 Da

HIF-1-alpha Transcription Factor Network Pathway (138045); Iron Uptake And Transport Pathway (187191); Metal Ion SLC Transporters Pathway (161066); Porphyrin And Chlorophyll Metabolism Pathway (83021); Porphyrin And Chlorophyll Metabolism Pathway (407); SLC-mediated Transmembrane Transport Pathway (119558); Transmembrane Transport Of Small Molecules Pathway (106572); Transport Of Glucose And Other Sugars, Bile Salts And Organic Acids, Metal Ions And Amine Compounds Pathway (119571)

The protein encoded by this gene is a metalloprotein that binds most of the copper in plasma and is involved in the peroxidation of Fe(II)transferrin to Fe(III) transferrin. Mutations in this gene cause aceruloplasminemia, which results in iron accumulation and tissue damage, and is associated with diabetes and neurologic abnormalities. [provided by RefSeq]

Function: Ceruloplasmin is a blue, copper-binding (6-7 atoms per molecule) glycoprotein. It has ferroxidase activity oxidizing Fe2+ to Fe3+ without releasing radical oxygen species. It is involved in iron transport across the cell membrane. Catalytic activity: 4 Fe2+ + 4 H+ + O2 = 4 Fe3+ + 2 H2O. Cofactor: Binds 6 copper ions per monomer. Subcellular location: Secreted. Tissue specificity: Expressed by the liver and secreted in plasma. Involvement in disease: Defects in CP are the cause of aceruloplasminemia (ACERULOP) [. MIM:604290]. It is an autosomal recessive disorder of iron metabolism characterized by iron accumulation in the brain as well as visceral organs. Clinical features consist of the triad of retinal degeneration, diabetes mellitus and neurological disturbances.Note=Ceruloplasmin levels are decreased in Wilson disease, in which copper cannot be incorporated into ceruloplasmin in liver because of defects in the copper-transporting ATPase 2. Sequence similarities: Belongs to the multicopper oxidase family.Contains 3 F5/8 type A domains.Contains 6 plastocyanin-like domains.

48-Strip-Wells

470 USD

96-Strip-Wells

675