Mouse Cathepsin D ELISA Kit | MyBioSource MBS723728 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse Cathepsin D ELISA Kit

catalog :

MBS723728

quantity :

48-Strip-Wells

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS723728

products type :

ELISA Kit

products full name :

Mouse Cathepsin D ELISA Kit

products short name :

Cathepsin D

other names :

CTSD; Cathepsin D; cathepsin D; lysosomal aspartyl protease; lysosomal aspartyl peptidase; ceroid-lipofuscinosis, neuronal 10; epididymis secretory sperm binding protein Li 130P; cathepsin D

products gene name :

CTSD

other gene names :

CTSD; CTSD; CPSD; CLN10; HEL-S-130P; CPSD

uniprot entry name :

CATD_HUMAN

reactivity :

Mouse

specificity :

This assay has high sensitivity and excellent specificity for detection of CTSD. No significant cross-reactivity or interference between CTSD and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CTSD and all the analogues, therefore, cross reaction may still exist in some cases.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 pg/mL.

other info2 :

Intended Uses: This CTSD ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse CTSD. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

products categories :

Neurobiology

products description :

Principle of the assay: CTSD ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CTSD antibody and an CTSD-HRP conjugate. The assay sample and buffer are incubated together with CTSD-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CTSD concentration since CTSD from samples and CTSD-HRP conjugate compete for the anti-CTSD antibody binding site. Since the number of sites is limited, as more sites are occupied by CTSD from the sample, fewer sites are left to bind CTSD-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CTSD concentration in each sample is interpolated from this standard curve.

ncbi gi num :

48146011

ncbi acc num :

CAG33228.1

uniprot acc num :

P07339

ncbi mol weight :

44,552 Da

ncbi pathways :

Adaptive Immune System Pathway (366160); Ceramide Signaling Pathway (138023); Collagen Degradation Pathway (730309); Degradation Of The Extracellular Matrix Pathway (576263); Direct P53 Effectors Pathway (137939); Extracellular Matrix Organization Pathway (576262); Immune System Pathway (106386); LKB1 Signaling Events Pathway (137957); Lysosome Pathway (99052); Lysosome Pathway (96865)

ncbi summary :

This gene encodes a lysosomal aspartyl protease composed of a dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor. This proteinase, which is a member of the peptidase C1 family, has a specificity similar to but narrower than that of pepsin A. Transcription of this gene is initiated from several sites, including one which is a start site for an estrogen-regulated transcript. Mutations in this gene are involved in the pathogenesis of several diseases, including breast cancer and possibly Alzheimer disease. [provided by RefSeq, Jul 2008]

uniprot summary :

CTSD: Acid protease active in intracellular protein breakdown. Involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease. Consists of a light chain and a heavy chain. Belongs to the peptidase A1 family. Protein type: Protease; EC 3.4.23.5; Motility/polarity/chemotaxis; Autophagy. Chromosomal Location of Human Ortholog: 11p15.5. Cellular Component: extracellular matrix; lysosomal lumen; extracellular space; lysosome; melanosome; extracellular region. Molecular Function: protein binding; aspartic-type endopeptidase activity. Biological Process: collagen catabolic process; extracellular matrix disassembly; extracellular matrix organization and biogenesis; antigen processing and presentation of exogenous peptide antigen via MHC class II; proteolysis. Disease: Ceroid Lipofuscinosis, Neuronal, 10

size1 :

48-Strip-Wells

price1 :

470 USD

size2 :

96-Strip-Wells

price2 :

675

more info or order :

MyBioSource product webpage