ELISA/assay

Human V Ha Ras Harvey Rat Sarcoma Viral Oncogene Homolog ELISA Kit

MBS723727

96 Strip Wells

675 USD

ELISA Kit

Human V Ha Ras Harvey Rat Sarcoma Viral Oncogene Homolog ELISA Kit

V Ha Ras Harvey Rat Sarcoma Viral Oncogene Homolog

HRAS, partial; GTPase HRas; GTPase HRas; p19 H-RasIDX protein; c-has/bas p21 protein; transforming protein p21; Ha-Ras1 proto-oncoprotein; c-ras-Ki-2 activated oncogene; GTP- and GDP-binding peptide B; transformation gene: oncogene HAMSV; Harvey rat sarcoma viral oncoprotein; Ras family small GTP binding protein H-Ras; v-Ha-ras Harvey rat sarcoma viral oncogene homolog; Harvey rat sarcoma viral oncogene homolog; H-Ras-1; Ha-Ras; Transforming protein p21; c-H-ras; p21rasCleaved into the following chain:GTPase HRas, N-terminally processed

HRAS

HRAS; HRAS; CTLO; HAMSV; HRAS1; RASH1; p21ras; C-H-RAS; H-RASIDX; C-BAS/HAS; C-HA-RAS1; HRAS1

RASH_HUMAN

Human

Store all reagents at 2-8 degree C.

Cancer

Intended Uses: This HRAS ELISA kit is intended Laboratory for research use only and is not for use in diagnostic or therapeutic procedures.The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration ofHRAS in the sample, thisHRAS ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versusHRAS concentration. The concentration ofHRAS in the samples is then determined by comparing the O.D. of the samples to the standard curve. Principle of the Assay: This HRAS enzyme linked immunosorbent assay applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific forHRAS. Standards or samples are then added to the microtiter plate wells andHRAS if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount ofHRAS present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific forHRAS are added to each well to "sandwich" theHRAS immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, A and B substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period.Only those wells that containHRAS and enzyme-conjugated antibody will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450 nm.

49457536

CAG47067.1

P01112

18,870 Da

ARMS-mediated Activation Pathway 106466!!Activation Of NMDA Receptor Upon Glutamate Binding And Postsynaptic Events Pathway 161033!!Activation Of RAS In B Cells Pathway 576252!!Acute Myeloid Leukemia Pathway 83117!!Acute Myeloid Leukemia Pathway 529!!Adaptive Immune System Pathway 366160!!Alcoholism Pathway 585563!!Alcoholism Pathway 587116!!Axon Guidance Pathway 83065!!Axon Guidance Pathway 476

This gene belongs to the Ras oncogene family, whose members are related to the transforming genes of mammalian sarcoma retroviruses. The products encoded by these genes function in signal transduction pathways. These proteins can bind GTP and GDP, and they have intrinsic GTPase activity. This protein undergoes a continuous cycle of de- and re-palmitoylation, which regulates its rapid exchange between the plasma membrane and the Golgi apparatus. Mutations in this gene cause Costello syndrome, a disease characterized by increased growth at the prenatal stage, growth deficiency at the postnatal stage, predisposition to tumor formation, mental retardation, skin and musculoskeletal abnormalities, distinctive facial appearance and cardiovascular abnormalities. Defects in this gene are implicated in a variety of cancers, including bladder cancer, follicular thyroid cancer, and oral squamous cell carcinoma. Multiple transcript variants, which encode different isoforms, have been identified for this gene. [provided by RefSeq, Jul 2008]

96 Strip Wells

675 USD