Mouse Arachidonate 15-lipoxygenase B (ALOX15B) ELISA Kit | MyBioSource MBS7237252 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse Arachidonate 15-lipoxygenase B (ALOX15B) ELISA Kit

catalog :

MBS7237252

quantity :

48-Strip-Wells

price :

440 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS7237252

products type :

ELISA Kit

products full name :

Mouse Arachidonate 15-lipoxygenase B (ALOX15B) ELISA Kit

products short name :

[Arachidonate 15-lipoxygenase B (ALOX15B)]

other names :

[arachidonate 15-lipoxygenase B; Arachidonate 15-lipoxygenase B; arachidonate 15-lipoxygenase B; 15-LOX-2; 15-LOX-B; 15-lipoxygenase 2; linoleate 13-lipoxygenase 15-LOb; arachidonate 15-lipoxygenase type II; arachidonate 15-lipoxygenase, second type; arachidonate 15-lipoxygenase, type B; 15-lipoxygenase 2; 15-LOX-2; Arachidonate 15-lipoxygenase type II; Linoleate 13-lipoxygenase 15-LOb (EC:1.13.11.-)]

products gene name :

[ALOX15B]

other gene names :

[Alox15b; Alox15b; 15-LOX-B; 15-LOX-2]

uniprot entry name :

LX15B_RAT

reactivity :

Mouse

specificity :

This assay has high sensitivity and excellent specificity for detection of ALOX15B. No significant cross-reactivity or interference between ALOX15B and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ALOX15B and all the analogues, therefore, cross reaction may still exist in some cases.

storage stability :

Store all reagents at 2-8 degree C.

image1 heading :

Typical Standard Curve/Testing Data

other info1 :

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Detection Range: 1.0-25ng/mL. Sensitivity: 0.1ng/mL

products description :

Intended Uses: This ALOX15B ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse ALOX15B. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: ALOX15B ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ALOX15B antibody and an ALOX15B-HRP conjugate. The assay sample and buffer are incubated together with ALOX15B-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ALOX15B concentration since ALOX15B from samples and ALOX15B-HRP conjugate compete for the anti-ALOX15B antibody binding site. Since the number of sites is limited, as more sites are occupied by ALOX15B from the sample, fewer sites are left to bind ALOX15B-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ALOX15B concentration in each sample is interpolated from this standard curve.

ncbi gi num :

23463277

ncbi acc num :

NP_695213.1

ncbi gb acc num :

NM_153301.2

ncbi mol weight :

76,145 Da

ncbi pathways :

Arachidonic Acid Metabolism Pathway (83383); Arachidonic Acid Metabolism Pathway (366); Eicosanoid Synthesis Pathway (198497); Metabolic Pathways (132933); Serotonergic Synapse Pathway (525343)

ncbi summary :

human homolog is an epidermis-type lipoxygenase and member of the multigene family of mammalian lipoxygenases [RGD, Feb 2006]

uniprot summary :

ALOX15B: Converts arachidonic acid exclusively to 15S- hydroperoxyeicosatetraenoic acid, while linoleic acid is less well metabolized. Belongs to the lipoxygenase family. 4 isoforms of the human protein are produced by alternative splicing. Protein type: Lipid Metabolism - arachidonic acid; Motility/polarity/chemotaxis; Cell cycle regulation; EC 1.13.11.33; Apoptosis; Oxidoreductase. Cellular Component: cytoskeleton; extrinsic to membrane; membrane; cytoplasm; plasma membrane; intracellular; nucleus; cytosol. Molecular Function: arachidonate 15-lipoxygenase activity; lipoxygenase activity; iron ion binding; calcium ion binding; lipid binding. Biological Process: negative regulation of cell proliferation; lipoxygenase pathway; linoleic acid metabolic process; arachidonic acid metabolic process; hepoxilin biosynthetic process; negative regulation of growth; positive regulation of keratinocyte differentiation; negative regulation of cell cycle

size1 :

48-Strip-Wells

price1 :

440 USD

size2 :

96-Strip-Wells

price2 :

640

size3 :

5x96-Strip-Wells

price3 :

2895

size4 :

10x96-Strip-Wells

price4 :

5415

more info or order :

MyBioSource product webpage