ELISA/assay

Rat Epidermal growth factor ELISA Kit

MBS723633

48-Strip-Wells

470 USD

ELISA Kit

Rat Epidermal growth factor ELISA Kit

Epidermal growth factor

Rat Epidermal growth factor (EGF) ELISA Kit; Rat Epidermal growth factor ELISA Kit; Epidermal growth factor; Epidermal growth factor (Rat)

epidermal growth factor

EGF

Rat

This assay has high sensitivity and excellent specificity for detection of EGF. No significant cross-reactivity or interference between EGF and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between EGF and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Sandwich. Sensitivity: 1.0 pg/mL.

Intended Uses: This EGF ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat EGF. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Rat ELISA Kit

Principle of the Assay: EGF ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for EGF. Standards or samples are then added to the microtiter plate wells and EGF if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of EGF present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for EGF are added to each well to "sandwich" the EGF immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain EGF and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The EGF concentration in each sample is interpolated from this standard curve.

688072

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48-Strip-Wells

470 USD

96-Strip-Wells

675