Mouse Myosin-11 (MYH11) ELISA Kit | MyBioSource MBS7236295 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse Myosin-11 (MYH11) ELISA Kit

catalog :

MBS7236295

quantity :

48-Strip-Wells

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS7236295

products type :

ELISA Kit

products full name :

Mouse Myosin-11 (MYH11) ELISA Kit

products short name :

Myosin-11 (MYH11)

other names :

myosin-11 isoform 2; Myosin-11; myosin-11; myosin heavy chain 11, smooth muscle; myosin heavy chain, smooth muscle isoform; myosin, heavy polypeptide 11, smooth muscle; Myosin heavy chain 11; Myosin heavy chain, smooth muscle isoform; SMMHC

products gene name :

MYH11

other gene names :

Myh11; Myh11; SM1; SM2; smMHC; AV071570

uniprot entry name :

MYH11_MOUSE

reactivity :

Mouse

specificity :

This assay has high sensitivity and excellent specificity for detection of MYO-11. No significant cross-reactivity or interference between MYO-11 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between MYO-11 and all the analogues, therefore, cross reaction may still exist in some cases.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

other info2 :

Intended Uses: This MYO-11 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse MYO-11. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

products categories :

Signal Transduction

products description :

Principle of the assay: MYO-11 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-MYO-11 antibody and an MYO-11-HRP conjugate. The assay sample and buffer are incubated together with MYO-11-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MYO-11 concentration since MYO-11 from samples and MYO-11-HRP conjugate compete for the anti-MYO-11 antibody binding site. Since the number of sites is limited, as more sites are occupied by MYO-11 from the sample, fewer sites are left to bind MYO-11-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MYO-11 concentration in each sample is interpolated from this standard curve.

ncbi gi num :

241982718

ncbi acc num :

NP_001155247.1

ncbi gb acc num :

NM_001161775.1

ncbi mol weight :

223,266 Da

ncbi pathways :

Axon Guidance Pathway (970585); Developmental Biology Pathway (970813); EPH-Ephrin Signaling Pathway (1000783); EPHA-mediated Growth Cone Collapse Pathway (1000784); Muscle Contraction Pathway (971218); Sema4D In Semaphorin Signaling Pathway (1000759); Sema4D Induced Cell Migration And Growth-cone Collapse Pathway (1000761); Semaphorin Interactions Pathway (1000758); Smooth Muscle Contraction Pathway (971207); Tight Junction Pathway (83268)

uniprot summary :

MYH11: Muscle contraction. A chromosomal aberration involving MYH11 is found in acute myeloid leukemia of M4EO subtype. Pericentric inversion inv(16)(p13;q22). The inversion produces a fusion protein consisting of the 165 N-terminal residues of CBF-beta (PEPB2) and the tail region of MYH11. Defects in MYH11 are the cause of familial aortic aneurysm thoracic type 4 (AAT4); also known as familial thoracic aortic aneurysm and dissection (TAAD). Aneurysms and dissections of the aorta usually result from degenerative changes in the aortic wall. Thoracic aortic aneurysms and dissections are primarily associated with a characteristic histologic appearance known as medial necrosis or Erdheim cystic medial necrosis in which there is degeneration and fragmentation of elastic fibers, loss of smooth muscle cells, and an accumulation of basophilic ground substance. Patients with AAT4 show marked aortic stiffness. Pathological aortas show large areas of medial degeneration with very low smooth muscle cells content. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Oncoprotein; Motor; Motility/polarity/chemotaxis. Cellular Component: smooth muscle contractile fiber; cytoplasm; stress fiber; muscle myosin complex; myosin complex. Molecular Function: calmodulin binding; structural constituent of muscle; motor activity; nucleotide binding; actin binding; ATP binding. Biological Process: elastic fiber assembly; smooth muscle contraction; cardiac muscle fiber development

size1 :

48-Strip-Wells

price1 :

470 USD

size2 :

96-Strip-Wells

price2 :

675

more info or order :

MyBioSource product webpage