ELISA/assay

Human Inducible nitric oxide synthase ELISA Kit

MBS723617

48-Strip-Wells

470 USD

ELISA Kit

Human Inducible nitric oxide synthase ELISA Kit

Inducible nitric oxide synthase

inos; Inositol-3-phosphate synthase; CG11143 gene product from transcript CG11143-RA; CG11143-PA; Inos-PA; inos; myo-inositol-1-phosphate-synthase; Myo-inositol 1-phosphate synthase; IPS; MI-1-P synthase

iNOS

Inos; Inos; bs36h12.y1; CG11143; DmelCG11143; INOS; CG11143; MIP synthase; IPS; MI-1-P synthase

INO1_DROME

Human

This assay has high sensitivity and excellent specificity for detection of iNOS. No significant cross-reactivity or interference between iNOS and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between iNOS and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Sandwich. Sensitivity: 0.1 ng/mL.

Neurobiology

Intended Uses: This iNOS ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human iNOS. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay INOS ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for iNOS. Standards or samples are then added to the microtiter plate wells and iNOS if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of iNOS present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for iNOS are added to each well to "sandwich" the iNOS immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain iNOS and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The iNOS concentration in each sample is interpolated from this standard curve.

17137626

NP_477405.1

NM_058057.6

62,249 Da

Inositol Phosphate Metabolism Pathway (3024); Inositol Phosphate Metabolism Pathway (1006205); Inositol Phosphate Metabolism Pathway (362); Metabolic Pathways (132021); Metabolism Pathway (1006204); Synthesis Of IP2, IP, And Ins In The Cytosol Pathway (1006210)

48-Strip-Wells

470 USD

96-Strip-Wells

675