ELISA/assay

Guinea Pig Apoptosis-related genes ELISA Kit

MBS723610

48-Strip-Wells

470 USD

ELISA Kit

Guinea Pig Apoptosis-related genes ELISA Kit

Apoptosis-related genes

Guinea Pig Apoptosis-related genes ELISA Kit; Apoptosis-related genes; Apoptosis-related genes ELISA Kit (Guinea Pig)

apoptosis-related protein, partial

GuineaPig

Sensitivity: The sensitivity in this assay is 0.1 ng/mL. Specificity: This assay has high sensitivity and excellent specificity for detection of ARG. No significant cross-reactivity or interference between ARG and analogues was observed.

Store all reagents at 2-8 degree C

Samples: Cell culture fluid, body fluid, tissue homogenate, serum and blood plasma

Guinea Pig ELISA Kit

For Samples: Cell culture fluid, body fluid, tissue homogenate, serum and blood plasma. Intended Uses: This ARG ELISA kit is intended for laboratory research use only and not for use in diagnostic or therapeutic procedures. The stop solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of ARG in the sample, this ARG ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus ARG concentration. The concentration of in the samples is then determined by comparing the O.D. of the samples to the standard curve. Principle of the Assay: The coated well immunoenzymatic assay for the quantitative measurement of ARG utilizes a multiclonal anti-ARG antibody and an ARG-HRP conjugate. The assay sample and buffer are incubated together with ARG-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ARG concentration since ARG from samples and ARG-HRP conjugate compete for the anti-ARG antibody binding site. Since the number of sites is limited, as more sites are occupied by ARG from the sample, fewer sites are left to bind ARG-HRP conjugate. Standards of known ARG concentrations are run concurrently with the samples being assayed and a standard curve is plotted relating the intensity of the color (Optical Density) to the concentration of ARG. The ARG concentration in each sample is interpolated from this standard curve.

45269013

AAS55919.1

Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Salmonella Infection Pathway (375172); Salmonella Infection Pathway (375149)

48-Strip-Wells

470 USD

96-Strip-Wells

675