ELISA/assay

Mouse Amyloid beta Protein 42 ELISA Kit

MBS723597

96 Strip Wells

675 USD

ELISA Kit

Mouse Amyloid beta Protein 42 ELISA Kit

Amyloid beta Protein 42

Mouse Amyloid b Protein 42 ELISA Kit

Amyloid beta A4 protein; Amyloid beta A4 protein; amyloid beta A4 protein; preA4; protease nexin-II; peptidase nexin-II; beta-amyloid peptide; beta-amyloid peptide(1-40); beta-amyloid peptide(1-42); alzheimer disease amyloid protein; cerebral vascular amyloid peptide; amyloid beta (A4) precursor protein; ABPP; APPI; APP; Alzheimer disease amyloid protein; Cerebral vascular amyloid peptide; CVAP; PreA4; Protease nexin-II; PN-II

Abeta42

Ab42

APP; APP; AAA; AD1; PN2; ABPP; APPI; CVAP; ABETA; PN-II; CTFgamma; A4; AD1; APP; CVAP; PN-II; S-APP-alpha; S-APP-beta; AICD-59; AID(59); AICD-57; AID(57); AICD-50; AID(50)

A4_HUMAN

Mouse

Store all reagents at 2-8 degree C.

Sensitivity: The sensitivity in this assay is 1.0 pg/mL.

Neurobiology

For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate . INTENDED USE This A? 42 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse A? 42. This ELISA kit for research use only, not for therapeutic or diagnostic applications! . PRINCIPLE OF THE ASSAY A? 42 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-A? 42 antibody and an A? 42-HRP conjugate. The assay sample and buffer are incubated together with A? 42-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the A? 42 concentration since A? 42 from samples and A? 42-HRP conjugate compete for the anti-A? 42 antibody binding site. Since the number of sites is limited, as more sites are occupied by A? 42 from the sample, fewer sites are left to bind A? 42-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The A? 42 concentration in each sample is interpolated from this standard curve.

112927

P05067.3

84,521 Da

Activated TLR4 Signalling Pathway 106400!!Advanced Glycosylation Endproduct Receptor Signaling Pathway 187092!!Alzheimer's Disease Pathway 83097!!Alzheimer's Disease Pathway 509!!Alzheimers Disease Pathway 672448!!Amyloids Pathway 366238!!Caspase Cascade In Apoptosis Pathway 137974!!Class A/1 (Rhodopsin-like Receptors) Pathway 106357!!Cytosolic Sensors Of Pathogen-associated DNA Pathway 576255!!DEx/H-box Helicases Activate Type I IFN And Inflammatory Cytokines Production Pathway 833822

This gene encodes a cell surface receptor and transmembrane precursor protein that is cleaved by secretases to form a number of peptides. Some of these peptides are secreted and can bind to the acetyltransferase complex APBB1/TIP60 to promote transcriptional activation, while others form the protein basis of the amyloid plaques found in the brains of patients with Alzheimer disease. In addition, two of the peptides are antimicrobial peptides, having been shown to have bacteriocidal and antifungal activities. Mutations in this gene have been implicated in autosomal dominant Alzheimer disease and cerebroarterial amyloidosis (cerebral amyloid angiopathy). Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq, Aug 2014]

96 Strip Wells

675 USD