Mouse Alkaline ceramidase 1 (ACER1) ELISA Kit | MyBioSource MBS7235867 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse Alkaline ceramidase 1 (ACER1) ELISA Kit

catalog :

MBS7235867

quantity :

48-Strip-Wells

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS7235867

products type :

ELISA Kit

products full name :

Mouse Alkaline ceramidase 1 (ACER1) ELISA Kit

products short name :

Alkaline ceramidase 1 (ACER1)

other names :

alkaline ceramidase 1; Alkaline ceramidase 1; alkaline ceramidase 1; alkCDase 1; alkaline CDase 1; acylsphingosine deacylase 3; N-acylsphingosine amidohydrolase 3; N-acylsphingosine amidohydrolase (alkaline ceramidase) 3; alkaline ceramidase 1; Acylsphingosine deacylase 3; N-acylsphingosine amidohydrolase 3

products gene name :

ACER1

other gene names :

ACER1; ACER1; ASAH3; ALKCDase1; ASAH3; AlkCDase 1; Alkaline CDase 1

uniprot entry name :

ACER1_HUMAN

reactivity :

Mouse

specificity :

This assay has high sensitivity and excellent specificity for detection of ACER1. No significant cross-reactivity or interference between ACER1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ACER1 and all the analogues, therefore, cross reaction may still exist in some cases.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

other info2 :

Intended Uses: This ACER1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse ACER1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

products description :

Principle of the assay: ACER1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ACER1 antibody and an ACER1-HRP conjugate. The assay sample and buffer are incubated together with ACER1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ACER1 concentration since ACER1 from samples and ACER1-HRP conjugate compete for the anti-ACER1 antibody binding site. Since the number of sites is limited, as more sites are occupied by ACER1 from the sample, fewer sites are left to bind ACER1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ACER1 concentration in each sample is interpolated from this standard curve.

ncbi gi num :

19424128

ncbi acc num :

NP_597999.1

ncbi gb acc num :

NM_133492.2

ncbi mol weight :

31,095 Da

ncbi pathways :

Metabolic Pathways (132956); Metabolism Pathway (477135); Metabolism Of Lipids And Lipoproteins Pathway (160976); Sphingolipid De Novo Biosynthesis Pathway (530750); Sphingolipid Metabolism Pathway (82994); Sphingolipid Metabolism Pathway (119543); Sphingolipid Metabolism Pathway (369); Sphingosine Biosynthesis Pathway (524497); Sphingosine Biosynthesis Pathway (468292); Sphingosine And Sphingosine-1-phosphate Metabolism Pathway (545351)

ncbi summary :

Ceramides are synthesized during epidermal differentiation and accumulate within the interstices of the stratum corneum, where they represent critical components of the epidermal permeability barrier. Excess cellular ceramide can trigger antimitogenic signals and induce apoptosis, and the ceramide metabolites sphingosine and sphingosine-1-phosphate (S1P) are important bioregulatory molecules. Ceramide hydrolysis in the nucleated cell layers regulates keratinocyte proliferation and apoptosis in response to external stress. Ceramide hydrolysis also occurs at the stratum corneum, releasing free sphingoid base that functions as an endogenous antimicrobial agent. ACER1 is highly expressed in epidermis and catalyzes the hydrolysis of very long chain ceramides to generate sphingosine (Houben et al., 2006 [PubMed 16477081]; Sun et al., 2008 [PubMed 17713573]).[supplied by OMIM, Jul 2010]

uniprot summary :

ACER1: Hydrolyzes the sphingolipid ceramide into sphingosine and free fatty acid at an optimal pH of 8.0. Has a highly restricted substrate specificity for the natural stereoisomer of ceramide with D-erythro-sphingosine but not D-ribo- phytosphingosine or D-erythro-dihydrosphingosine as a backbone. May have a role in regulating the levels of bioactive lipids ceramide and sphingosine 1-phosphate, as well as complex sphingolipids. Belongs to the alkaline ceramidase family. Protein type: Hydrolase; Lipid Metabolism - sphingolipid; EC 3.5.1.23; Endoplasmic reticulum; Membrane protein, multi-pass; Membrane protein, integral. Chromosomal Location of Human Ortholog: 19p13.3. Cellular Component: endoplasmic reticulum membrane; endoplasmic reticulum; integral to membrane. Molecular Function: ceramidase activity. Biological Process: keratinocyte differentiation; epidermis development; regulation of lipid metabolic process; sphingolipid metabolic process; response to alkalinity; sphingolipid biosynthetic process; sphingosine biosynthetic process; ceramide catabolic process; cell differentiation

size1 :

48-Strip-Wells

price1 :

470 USD

size2 :

96-Strip-Wells

price2 :

675

more info or order :

MyBioSource product webpage