ELISA/assay

Rat Nephrin ELISA Kit

MBS723583

96 Strip Wells

675 USD

ELISA Kit

Rat Nephrin ELISA Kit

Nephrin

nephrin; Nephrin; nephrin; renal glomerulus-specific cell adhesion receptor; nephrosis 1, congenital, Finnish type (nephrin); Renal glomerulus-specific cell adhesion receptor

Nephrin

NPHS1; NPHS1; CNF; NPHN; nephrin; NPHN

NPHN_HUMAN

Rat

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Intended Uses: This Nephrin ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat Nephrin. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Sensitivity: The sensitivity in this assay is 0.1 ng/mL.

Principle of the Assay: Nephrin ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-Nephrin antibody and an Nephrin-HRP conjugate. The assay sample and buffer are incubated together with Nephrin-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Nephrin concentration since Nephrin from samples and Nephrin-HRP conjugate compete for the anti-Nephrin antibody binding site. Since the number of sites is limited, as more sites are occupied by Nephrin from the sample, fewer sites are left to bind Nephrin-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Nephrin concentration in each sample is interpolated from this standard curve.

10441644

AAG17141.1

130,546 Da

Cell-Cell Communication Pathway 477132!!Nephrin Interactions Pathway 187228!!Nephrin/Neph1 Signaling In The Kidney Podocyte Pathway 138072

This gene encodes a member of the immunoglobulin family of cell adhesion molecules that functions in the glomerular filtration barrier in the kidney. The gene is primarily expressed in renal tissues, and the protein is a type-1 transmembrane protein found at the slit diaphragm of glomerular podocytes. The slit diaphragm is thought to function as an ultrafilter to exclude albumin and other plasma macromolecules in the formation of urine. Mutations in this gene result in Finnish-type congenital nephrosis 1, characterized by severe proteinuria and loss of the slit diaphragm and foot processes.[provided by RefSeq, Oct 2009]

96 Strip Wells

675 USD