catalog number :
MBS723535
products type :
ELISA Kit
products full name :
Rabbit Fructose-1, 6-bisphosphate aldolase ELISA Kit
products short name :
Fructose-1, 6-bisphosphate aldolase
other names :
Fda; Fda; Fructose-bisphosphate aldolase; Fda; Fda
other gene names :
fda; fda; PPUBIRD1_4744
uniprot entry name :
E4R8G6_PSEPB
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.
other info2 :
Intended Uses: This ALDOA ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rabbit ALDOA. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
products description :
Principle of the Assay: ALDOA ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ALDOA antibody and an ALDOA-HRP conjugate. The assay sample and buffer are incubated together with ALDOA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ALDOA concentration since ALDOA from samples and ALDOA-HRP conjugate compete for the anti-ALDOA antibody binding site. Since the number of sites is limited, as more sites are occupied by ALDOA from the sample, fewer sites are left to bind ALDOA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ALDOA concentration in each sample is interpolated from this standard curve.
ncbi acc num :
ADR62302.1
ncbi mol weight :
37,937 Da
ncbi pathways :
Biosynthesis Of Amino Acids Pathway (792081); Biosynthesis Of Amino Acids Pathway (795174); Biosynthesis Of Secondary Metabolites Pathway (616511); Carbon Metabolism Pathway (816944); Carbon Metabolism Pathway (817567); Fructose And Mannose Metabolism Pathway (616433); Fructose And Mannose Metabolism Pathway (291); Glycolysis / Gluconeogenesis Pathway (616429); Glycolysis / Gluconeogenesis Pathway (287); Metabolic Pathways (616510)