ELISA/assay

Rat Adrenergic Receptor, Alpha 1A ELISA Kit

MBS723518

48-Strip-Wells

470 USD

ELISA Kit

Rat Adrenergic Receptor, Alpha 1A ELISA Kit

Adrenergic Receptor, Alpha 1A

alpha adrenergic receptor subtype alpha 1a; Alpha-1D adrenergic receptor; alpha-1D adrenergic receptor; alpha-1D adrenoceptor; alpha-1D adrenoreceptor; alpha-1A adrenergic receptor; alpha-1D-adrenergic receptor; alpha-adrenergic receptor 1a; adrenergic, alpha-1A-, receptor; adrenergic, alpha-1D-, receptor; adrenergic, alpha -1D-, receptor; adrenoceptor alpha 1D; Alpha-1A adrenergic receptor; Alpha-1D adrenoreceptor; Alpha-1D adrenoceptor; Alpha-adrenergic receptor 1a

ADRalpha1A

ADRA1D; ADRA1D; DAR; ADRA1; ADRA1A; ADRA1R; ALPHA1; dJ779E11.2; ADRA1A; Alpha-1D adrenoceptor

ADA1D_HUMAN

Rat

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, cell culture superna tants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 pg/mL.

Immunology

For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate . INTENDED USE This ADR?1A ELISA kit is a 1.5 hour solid-phase ELISA designed for thequantitative determination of Rat ADR?1A. This ELISA kit for research use only, not for therapeutic or diagnostic applications! . PRINCIPLE OF THE ASSAY ADR?1A ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ADR?1A antibody and an ADR?1A-HRP conjugate. The assay sample and buffer are incubated together with ADR?1A-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ADR?1A concentration since ADR?1A from samples and ADR?1A-HRP conjugate compete for the anti-ADR?1A antibody binding site. Since the number of sites is limited, as more sites are occupied by ADR?1A from the sample, fewer sites are left to bind ADR?1A-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ADR?1A concentration in each sample is interpolated from this standard curve.

7690135

AAB31163.2

60,463 Da

Adrenergic Signaling In Cardiomyocytes Pathway (908257); Adrenergic Signaling In Cardiomyocytes Pathway (909696); Adrenoceptors Pathway (106365); Amine Ligand-binding Receptors Pathway (106363); Calcium Regulation In The Cardiac Cell Pathway (198906); Calcium Signaling Pathway (83050); Calcium Signaling Pathway (459); Class A/1 (Rhodopsin-like Receptors) Pathway (106357); G Alpha (12/13) Signalling Events Pathway (106041); G Alpha (q) Signalling Events Pathway (106043)

Alpha-1-adrenergic receptors (alpha-1-ARs) are members of the G protein-coupled receptor superfamily. They activate mitogenic responses and regulate growth and proliferation of many cells. There are 3 alpha-1-AR subtypes: alpha-1A, -1B and -1D, all of which signal through the Gq/11 family of G-proteins and different subtypes show different patterns of activation. This gene encodes alpha-1D-adrenergic receptor. Similar to alpha-1B-adrenergic receptor gene, this gene comprises 2 exons and a single intron that interrupts the coding region. [provided by RefSeq, Jul 2008]

ADRA1D: This alpha-adrenergic receptor mediates its effect through the influx of extracellular calcium. Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRA1D sub-subfamily. Protein type: Membrane protein, multi-pass; Membrane protein, integral; GPCR, family 1; Receptor, GPCR. Chromosomal Location of Human Ortholog: 20p13. Cellular Component: integral to plasma membrane; plasma membrane. Molecular Function: alpha1-adrenergic receptor activity. Biological Process: G-protein signaling, coupled to cAMP nucleotide second messenger; G-protein coupled receptor protein signaling pathway; cell proliferation; cell-cell signaling; multicellular organismal development; positive regulation of cell proliferation; negative regulation of the force of heart contraction during baroreceptor response to increased systemic arterial blood pressure; norepinephrine-epinephrine vasoconstriction involved in regulation of systemic arterial blood pressure; DNA metabolic process

48-Strip-Wells

470 USD

96-Strip-Wells

675