ELISA/assay

Human Tau Protein ELISA Kit

MBS723516

48-Strip-Wells-(Competitive)

440 USD

ELISA Kit

Human Tau Protein ELISA Kit

[Tau Protein]

[tau, isoform A; Microtubule-associated protein; CG45110 gene product from transcript CG45110-RH; CG45110-PA; CG45110-PB; CG45110-PC; CG45110-PE; CG45110-PF; CG45110-PG; CG45110-PH; CG45110-PI; CG45110-PJ; CG45110-PK; CG45110-PL; CG45110-PM; tau; tau-PA; tau-PB; tau-PC; tau-PE; tau-PF; tau-PG; tau-PH; tau-PI; tau-PJ; tau-PK; tau-PL; tau-PM]

[Tau]

[tau; tau; BcDNA:RE16764; CG12881; CG31057; CG31058; CG34294; CG45110; CG5606; d-tau; DmelCG45110; dtau; dTau; Tau; CG34294; Dmel_CG31057]

Q9VB13_DROME

Human

This assay has high sensitivity and excellent specificity for detection of TAU. No significant cross-reactivity or interference between TAU and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between TAU and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive or Sandwich. Detection Range: 0.5-10ng/mL. Sensitivity: 0.1ng/mL

Neurobiology

Intended Uses This TAU ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human TAU. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: TAU ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for TAU. Standards or samples are then added to the microtiter plate wells and TAU if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of TAU present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for TAU are added to each well to "sandwich” the TAU immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain TAU and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TAU concentration in each sample is interpolated from this standard curve.

21357661

NP_651575.1

NM_143318.3

38,538 Da

Apoptosis Pathway (1005319); Apoptotic Cleavage Of Cellular Proteins Pathway (1005323); Apoptotic Execution Phase Pathway (1005322); Caspase-mediated Cleavage Of Cytoskeletal Proteins Pathway (1005324)

48-Strip-Wells-(Competitive)

440 USD

48-Strip-Wells-(Sandwich)

440

96-Strip-Wells-(Competitive)

640

96-Strip-Wells-(Sandwich)

640

5x96-Strip-Wells-(Competitive)

2895

5x96-Strip-Wells-(Sandwich)

2895

10x96-Strip-Wells-(Competitive)

5415

10x96-Strip-Wells-(Sandwich)

5415