ELISA/assay

Mouse Cholesterol 24-hydroxylase (CYP46A1) ELISA Kit

MBS7235102

48-Strip-Wells

470 USD

ELISA Kit

Mouse Cholesterol 24-hydroxylase (CYP46A1) ELISA Kit

Cholesterol 24-hydroxylase (CYP46A1)

cholesterol 24-hydroxylase; Cholesterol 24-hydroxylase; cholesterol 24-hydroxylase; CH24H; cytochrome P450 46A1; cytochrome P450, subfamily 46 (cholesterol 24-hydroxylase); cytochrome P450, family 46, subfamily A, polypeptide 1; Cytochrome P450 46A1

CYP46A1

CYP46A1; CYP46A1; CP46; CYP46; CYP46; CH24H

CP46A_HUMAN

Mouse

This assay has high sensitivity and excellent specificity for detection of CYP46A1. No significant cross-reactivity or interference between CYP46A1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CYP46A1 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Intended Uses: This CYP46A1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse CYP46A1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the assay: CYP46A1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CYP46A1 antibody and an CYP46A1-HRP conjugate. The assay sample and buffer are incubated together with CYP46A1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CYP46A1 concentration since CYP46A1 from samples and CYP46A1-HRP conjugate compete for the anti-CYP46A1 antibody binding site. Since the number of sites is limited, as more sites are occupied by CYP46A1 from the sample, fewer sites are left to bind CYP46A1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CYP46A1 concentration in each sample is interpolated from this standard curve.

5729796

NP_006659.1

NM_006668.1

38,315 Da

Bile Acid And Bile Salt Metabolism Pathway (106144); Biological Oxidations Pathway (105698); Cytochrome P450 - Arranged By Substrate Type Pathway (105700); Endogenous Sterols Pathway (105701); Metabolism Pathway (477135); Metabolism Of Lipids And Lipoproteins Pathway (160976); Phase 1 - Functionalization Of Compounds Pathway (105699); Primary Bile Acid Biosynthesis Pathway (82938); Primary Bile Acid Biosynthesis Pathway (299); Synthesis Of Bile Acids And Bile Salts Pathway (106145)

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum protein is expressed in the brain, where it converts cholesterol to 24S-hydroxycholesterol. While cholesterol cannot pass the blood-brain barrier, 24S-hydroxycholesterol can be secreted in the brain into the circulation to be returned to the liver for catabolism. [provided by RefSeq, Jul 2008]

CYP46A1: Involved in the turnover of cholesterol. It converts cholesterol into 24S-hydroxycholesterol and, to a lesser extent, 25-hydroxycholesterol. Belongs to the cytochrome P450 family. Protein type: Oxidoreductase; Lipid Metabolism - primary bile acid biosynthesis; EC 1.14.13.98; Membrane protein, integral. Chromosomal Location of Human Ortholog: 14q32.1. Cellular Component: endoplasmic reticulum membrane; endoplasmic reticulum; integral to membrane. Molecular Function: iron ion binding; cholesterol 24-hydroxylase activity; heme binding; steroid hydroxylase activity. Biological Process: bile acid biosynthetic process; nervous system development; bile acid metabolic process; xenobiotic metabolic process; cholesterol catabolic process; sterol metabolic process

48-Strip-Wells

470 USD

96-Strip-Wells

675