Mouse Carnitine palmitoyltransferase I ELISA Kit | MyBioSource MBS723506 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse Carnitine palmitoyltransferase I ELISA Kit

catalog :

MBS723506

quantity :

48-Strip-Wells

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS723506

products type :

ELISA Kit

products full name :

Mouse Carnitine palmitoyltransferase I ELISA Kit

products short name :

Carnitine palmitoyltransferase I

other names :

Carnitine O-palmitoyltransferase 1, liver isoform; Carnitine O-palmitoyltransferase 1, liver isoform; carnitine O-palmitoyltransferase 1, liver isoform; CPT I; CPT1-L; CPTI-L; carnitine palmitoyltransferase 1, liver; carnitine O-palmitoyltransferase I, liver isoform; Carnitine palmitoyltransferase 1 alpha, liver isoform; carnitine palmitoyltransferase 1a, liver; Carnitine O-palmitoyltransferase I, liver isoform; CPT I; CPTI-L; Carnitine palmitoyltransferase 1A

products gene name :

CPT-1

other gene names :

Cpt1a; Cpt1a; CPT-Ia; Cpt-1; Cpt1; CPT1-L; CPT I; CPTI-L

uniprot entry name :

CPT1A_RAT

reactivity :

Mouse

specificity :

This assay has high sensitivity and excellent specificity for detection of CPT-1. No significant cross-reactivity or interference between CPT-1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CPT-1 and all the analogues, therefore, cross reaction may still exist in some cases.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

other info2 :

Intended Uses: This CPT-1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse CPT-1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

products description :

Principle of the assay: CPT-1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CPT-1 antibody and an CPT-1-HRP conjugate. The assay sample and buffer are incubated together with CPT-1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CPT-1 concentration since CPT-1 from samples and CPT-1-HRP conjugate compete for the anti-CPT-1 antibody binding site. Since the number of sites is limited, as more sites are occupied by CPT-1 from the sample, fewer sites are left to bind CPT-1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CPT-1 concentration in each sample is interpolated from this standard curve.

ncbi gi num :

146345405

ncbi acc num :

P32198.2

ncbi mol weight :

88,125 Da

ncbi pathways :

AMPK Signaling Pathway (989832); AMPK Signaling Pathway (992181); Adipocytokine Signaling Pathway (83485); Adipocytokine Signaling Pathway (505); Beta Oxidation Meta Pathway (198526); Circadian Clock Pathway (1010370); Fatty Acid Beta Oxidation Pathway (219782); Fatty Acid Beta Oxidation 1 Pathway (198531); Fatty Acid Degradation Pathway (83328); Fatty Acid Degradation Pathway (296)

ncbi summary :

enzyme that catalyses the transfer of long chain fatty acids to carnitine for translocation across the mitochondrial inner membrane [RGD, Feb 2006]

uniprot summary :

CPT1A: Belongs to the carnitine/choline acetyltransferase family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Transferase; Lipid Metabolism - fatty acid; Mitochondrial; Membrane protein, multi-pass; Membrane protein, integral; EC 2.3.1.21. Cellular Component: mitochondrial outer membrane; endoplasmic reticulum membrane; intracellular membrane-bound organelle; membrane; mitochondrion; mitochondrial inner membrane; integral to membrane; integral to mitochondrial outer membrane. Molecular Function: identical protein binding; protein binding; carnitine O-palmitoyltransferase activity. Biological Process: response to drug; eating behavior; glucose metabolic process; long-chain fatty acid metabolic process; response to organic cyclic substance; positive regulation of fatty acid beta-oxidation; fatty acid beta-oxidation; triacylglycerol metabolic process; epithelial cell differentiation; transport; regulation of fatty acid oxidation; regulation of fatty acid beta-oxidation; carnitine metabolic process; regulation of insulin secretion; protein homooligomerization

size1 :

48-Strip-Wells

price1 :

470 USD

size2 :

96-Strip-Wells

price2 :

675

more info or order :

MyBioSource product webpage