catalog number :
MBS723494
products type :
ELISA Kit
products full name :
Rat Chemokine C-X-C-Motif Receptor 4 ELISA Kit
products short name :
Chemokine C-X-C-Motif Receptor 4
products gene name :
CXCR4
other gene names :
LOC443442
specificity :
This assay has high sensitivity and excellent specificity for detection of CXCR4. No significant cross-reactivity or interference between CXCR4 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CXCR4 and all the analogues, therefore, cross reaction may still exist in some cases.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 pg/mL.
other info2 :
Intended Uses: This CXCR4 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat CXCR4. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
products categories :
Immunology
products description :
Principle of the assay: CXCR4 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CXCR4 antibody and an CXCR4-HRP conjugate. The assay sample and buffer are incubated together with CXCR4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CXCR4 concentration since CXCR4 from samples and CXCR4-HRP conjugate compete for the anti-CXCR4 antibody binding site. Since the number of sites is limited, as more sites are occupied by CXCR4 from the sample, fewer sites are left to bind CXCR4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CXCR4 concentration in each sample is interpolated from this standard curve.
ncbi acc num :
NP_001009425.1
ncbi gb acc num :
NM_001009425.1
ncbi pathways :
Hematopoietic Cell Lineage Pathway (1030802); Hematopoietic Cell Lineage Pathway (489)