catalog number :
MBS723481
products type :
ELISA Kit
products full name :
Human Tenascin C ELISA Kit
products short name :
Tenascin C
other names :
tenascin-C, partial; Tenascin-C; Tenascin-C
uniprot entry name :
Q99857_HUMAN
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Assay Type: Competitive or Sandwich. Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Sensitivity: 1.0 ng/mL.
products categories :
Neurobiology
products description :
Intended Uses: This TNC ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human TNC. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: TNC ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-TNC antibody and an TNC-HRP conjugate. The assay sample and buffer are incubated together with TNC-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TNC concentration since TNC from samples and TNC-HRP conjugate compete for the anti-TNC antibody binding site. Since the number of sites is limited, as more sites are occupied by TNC from the sample, fewer sites are left to bind TNC-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TNC concentration in each sample is interpolated from this standard curve.
ncbi acc num :
AAB47574.1
ncbi mol weight :
9,764 Da
size1 :
48-Strip-Wells-(Competitive)
size2 :
48-Strip-Wells-(Sandwich)
size3 :
96-Strip-Wells-(Competitive)
size4 :
96-Strip-Wells-(Sandwich)