ELISA/assay

Human Anti Smooth Muscle Antibody ELISA Kit

MBS723459

96 Strip Wells

675 USD

ELISA Kit

Human Anti Smooth Muscle Antibody ELISA Kit

Anti Smooth Muscle Antibody

AsmA; AsmA; AsmA

ASMA

asmA; HRM2_42670

C0QDQ2_DESAH

Human

Store all reagents at 2-8 degree C.

Sensitivity: The sensitivity in this assay is 1.0U/ml.

Cardiovascular

For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate . INTENDED USE This ASMA ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ASMA. This ELISA kit for research use only, not for therapeutic or diagnostic applications! . PRINCIPLE OF THE ASSAY ASMA ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for ASMA. Standards or samples are then added to the microtiter plate wells and ASMA if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of ASMA present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for ASMA are added to each well to “sandwich” the ASMA immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain ASMA and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ASMA concentration in each sample is interpolated from this standard curve.

223694040

ACN17323.1

72,824 Da

96 Strip Wells

675 USD