Mouse Interferon beta ELISA Kit | MyBioSource MBS723426 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse Interferon beta ELISA Kit

catalog :

MBS723426

quantity :

48-Strip-Wells

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS723426

products type :

ELISA Kit

products full name :

Mouse Interferon beta ELISA Kit

products short name :

Interferon beta

products name syn :

Mouse Interferon b ELISA Kit

other names :

interferon beta; Interferon beta; interferon beta; IFN-beta; fibroblast interferon; interferon, beta 1, fibroblast; Fibroblast interferon

products gene name :

IFN-beta

products gene name syn :

IFN-b

other gene names :

IFNB1; IFNB1; IFB; IFF; IFNB; IFB; IFNB; IFN-beta

uniprot entry name :

IFNB_HUMAN

reactivity :

Mouse

specificity :

This assay has high sensitivity and excellent specificity for detection of IFNbeta. No significant cross-reactivity or interference between IFNbeta and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between IFNbeta and all the analogues, therefore, cross reaction may still exist in some cases.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Sandwich. Sensitivity: 1.0pg/mL.

products categories :

Cancer

products description :

Intended Uses This IFNbeta ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse IFNbeta. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: IFNbeta ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for IFNbeta. Standards or samples are then added to the microtiter plate wells and IFNbeta if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of IFNbeta present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for IFNbeta are added to each well to "sandwich” the IFNbeta immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain IFNbeta and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IFNbeta concentration in each sample is interpolated from this standard curve.

ncbi gi num :

4504603

ncbi acc num :

NP_002167.1

ncbi gb acc num :

NM_002176.2

ncbi mol weight :

22,294 Da

ncbi pathways :

Cellular Senescence Pathway (905991); Cellular Responses To Stress Pathway (645258); Chagas Disease (American Trypanosomiasis) Pathway (147809); Chagas Disease (American Trypanosomiasis) Pathway (147795); Cytokine Signaling In Immune System Pathway (366171); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Cytokines And Inflammatory Response Pathway (198794); Cytosolic DNA-sensing Pathway (125137); Cytosolic DNA-sensing Pathway (124833)

uniprot summary :

IFNB1: Has antiviral, antibacterial and anticancer activities. Belongs to the alpha/beta interferon family. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 9p21. Cellular Component: extracellular space; extracellular region. Molecular Function: interferon-alpha/beta receptor binding; cytokine activity. Biological Process: B cell proliferation; adaptive immune response; cytokine and chemokine mediated signaling pathway; response to virus; regulation of MHC class I biosynthetic process; natural killer cell activation; negative regulation of T cell differentiation; humoral immune response; T cell activation during immune response; cell surface receptor linked signal transduction; response to exogenous dsRNA; negative regulation of viral genome replication; B cell differentiation; defense response to bacterium; positive regulation of innate immune response; natural killer cell activation during immune response; innate immune response; positive regulation of transcription from RNA polymerase II promoter; blood coagulation; defense response to virus; positive regulation of peptidyl-serine phosphorylation of STAT protein; B cell activation during immune response

size1 :

48-Strip-Wells

price1 :

470 USD

size2 :

96-Strip-Wells

price2 :

675

more info or order :

MyBioSource product webpage