Mouse Biliverdin reductase A (BLVRA) ELISA Kit | MyBioSource MBS7233993 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse Biliverdin reductase A (BLVRA) ELISA Kit

catalog :

MBS7233993

quantity :

48-Strip-Wells

price :

440 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS7233993

products type :

ELISA Kit

products full name :

Mouse Biliverdin reductase A (BLVRA) ELISA Kit

products short name :

[Biliverdin reductase A (BLVRA)]

other names :

[biliverdin reductase A; Biliverdin reductase A; biliverdin reductase A; BVR A; biliverdin-IX alpha-reductase; biliverdin reductase A; Biliverdin-IX alpha-reductase]

products gene name :

[BLVRA]

other gene names :

[BLVRA; BLVRA; BVR; BLVR; BVRA; BLVR; BVR; BVR A]

uniprot entry name :

BIEA_HUMAN

reactivity :

Mouse

specificity :

This assay has high sensitivity and excellent specificity for detection of Blood Glucose. No significant cross-reactivity or interference between Blood Glucose and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between Blood Glucose and all the analogues, therefore, cross reaction may still exist in some cases.

storage stability :

Store all reagents at 2-8 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma. Assay Type: Quantitative Competitive. Sensitivity: 0.1 mmol/L.

other info2 :

Reactivity Note: No significant cross-reactivity or interference between this antigen and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection, therefore, cross reaction may still exist in some cases.

products description :

Intended Uses: This Blood Glucose ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat Blood Glucose. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: Blood Glucose ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-Blood Glucose antibody and an Blood Glucose-HRP conjugate. The assay sample and buffer are incubated together with Blood Glucose-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Blood Glucose concentration since Blood Glucose from samples and Blood Glucose-HRP conjugate compete for the anti-Blood Glucose antibody binding site. Since the number of sites is limited, as more sites are occupied by Blood Glucose from the sample, fewer sites are left to bind Blood Glucose-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Blood Glucose concentration in each sample is interpolated from this standard curve.

ncbi gi num :

359465572

ncbi acc num :

NP_001240752.1

ncbi gb acc num :

NM_001253823.1

ncbi mol weight :

33,428 Da

ncbi pathways :

Heme Degradation Pathway (160971); Metabolism Pathway (477135); Metabolism Of Porphyrins Pathway (106325); Porphyrin And Chlorophyll Metabolism Pathway (83021); Porphyrin And Chlorophyll Metabolism Pathway (407); Heme Degradation Pathway (545346); Heme Degradation Pathway (139483)

ncbi summary :

The protein encoded by this gene belongs to the biliverdin reductase family, members of which catalyze the conversion of biliverdin to bilirubin in the presence of NADPH or NADH. Mutations in this gene are associated with hyperbiliverdinemia. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Dec 2011]

uniprot summary :

BVR: Reduces the gamma-methene bridge of the open tetrapyrrole, biliverdin IX alpha, to bilirubin with the concomitant oxidation of a NADH or NADPH cofactor. Monomer. Liver. Belongs to the Gfo/Idh/MocA family. Biliverdin reductase subfamily. Protein type: Oxidoreductase; Kinase, protein; EC 1.3.1.24; Cofactor and Vitamin Metabolism - porphyrin and chlorophyll; Protein kinase, Ser/Thr (non-receptor); ATYPICAL group; BLVRA family. Chromosomal Location of Human Ortholog: 7p13. Cellular Component: cytosol. Molecular Function: zinc ion binding; biliverdin reductase activity. Biological Process: heme catabolic process; porphyrin metabolic process. Disease: Hyperbiliverdinemia

size1 :

48-Strip-Wells

price1 :

440 USD

size2 :

96-Strip-Wells

price2 :

640

size3 :

5x96-Strip-Wells

price3 :

2895

size4 :

10x96-Strip-Wells

price4 :

5415

more info or order :

MyBioSource product webpage