ELISA/assay

Mouse Neuregulin 1 ELISA Kit

MBS723388

48-Strip-Wells

470 USD

ELISA Kit

Mouse Neuregulin 1 ELISA Kit

Neuregulin 1

neuregulin 1, isoform CRA_k; Pro-neuregulin-1, membrane-bound isoform; pro-neuregulin-1, membrane-bound isoform; pro-NRG1; glial growth factor; neu differentiation factor; sensory and motor neuron derived factor; heregulin, alpha (45kD, ERBB2 p185-activator); neuregulin 1; Acetylcholine receptor-inducing activity; ARIA; Breast cancer cell differentiation factor p45; Glial growth factor; Heregulin; HRG; Neu differentiation factor; Sensory and motor neuron-derived factor

NRG-1

NRG1; NRG1; GGF; HGL; HRG; NDF; ARIA; GGF2; HRG1; HRGA; SMDF; MST131; MSTP131; NRG1-IT2; GGF; HGL; HRGA; NDF; SMDF; Pro-NRG1; ARIA; HRG

NRG1_HUMAN

Mouse

This assay has high sensitivity and excellent specificity for detection of NRG-1. No significant cross-reactivity or interference between NRG-1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between NRG-1 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 ng/mL.

Neurobiology

Intended Uses: This NRG-1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse NRG-1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: NRG-1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-NRG-1 antibody and an NRG-1-HRP conjugate. The assay sample and buffer are incubated together with NRG-1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the NRG-1 concentration since NRG-1 from samples and NRG-1-HRP conjugate compete for the anti-NRG-1 antibody binding site. Since the number of sites is limited, as more sites are occupied by NRG-1 from the sample, fewer sites are left to bind NRG-1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NRG-1 concentration in each sample is interpolated from this standard curve.

119583821

EAW63417.1

46,226 Da

Adaptive Immune System Pathway (366160); Cardiac Progenitor Differentiation Pathway (712094); Constitutive PI3K/AKT Signaling In Cancer Pathway (685535); DAP12 Interactions Pathway (685549); DAP12 Signaling Pathway (685550); Disease Pathway (530764); Downregulation Of ERBB2:ERBB3 Signaling Pathway (530740); Downstream Signal Transduction Pathway (106385); Downstream Signaling Events Of B Cell Receptor (BCR) Pathway (576250); Downstream Signaling Of Activated FGFR Pathway (160957)

The protein encoded by this gene is a membrane glycoprotein that that mediates cell-cell signaling and plays a critical role in the growth and development of multiple organ systems. An extraordinary variety of different isoforms are produced from this gene through alternative promoter usage and splicing. These isoforms are expressed in a tissue-specific manner and differ significantly in their structure, and are classified as types I, II, III, IV, V and VI. Dysregulation of this gene has been linked to diseases such as cancer, schizophrenia, and bipolar disorder (BPD). [provided by RefSeq, Jun 2014]

NRG1: Direct ligand for ERBB3 and ERBB4 tyrosine kinase receptors. Concomitantly recruits ERBB1 and ERBB2 coreceptors, resulting in ligand-stimulated tyrosine phosphorylation and activation of the ERBB receptors. The multiple isoforms perform diverse functions such as inducing growth and differentiation of epithelial, glial, neuronal, and skeletal muscle cells; inducing expression of acetylcholine receptor in synaptic vesicles during the formation of the neuromuscular junction; stimulating lobuloalveolar budding and milk production in the mammary gland and inducing differentiation of mammary tumor cells; stimulating Schwann cell proliferation; implication in the development of the myocardium such as trabeculation of the developing heart. Isoform 10 may play a role in motor and sensory neuron development. The cytoplasmic domain interacts with the LIM domain region of LIMK1. Interacts with ERBB3 and ERBB4. Type I isoforms are the predominant forms expressed in the endocardium. Isoform alpha is expressed in breast, ovary, testis, prostate, heart, skeletal muscle, lung, placenta liver, kidney, salivary gland, small intestine and brain, but not in uterus, stomach, pancreas, and spleen. Isoform 3 is the predominant form in mesenchymal cells and in non-neuronal organs, whereas isoform 6 is the major neuronal form. Isoform 8 is expressed in spinal cord and brain. Isoform 9 is the major form in skeletal muscle cells; in the nervous system it is expressed in spinal cord and brain. Also detected in adult heart, placenta, lung, liver, kidney, and pancreas. Isoform 10 is expressed in nervous system: spinal cord motor neurons, dorsal root ganglion neurons, and brain. Predominant isoform expressed in sensory and motor neurons. Not detected in adult heart, placenta, lung, liver, skeletal muscle, kidney, and pancreas. Not expressed in fetal lung, liver and kidney. Type IV isoforms are brain-specific. Belongs to the neuregulin family. 10 isoforms of the human protein are produced by alternative splicing. Protein type: Cell development/differentiation; Motility/polarity/chemotaxis; Ligand, receptor tyrosine kinase; Membrane protein, integral; Cytokine. Chromosomal Location of Human Ortholog: 8p12. Cellular Component: extracellular space; membrane; axon; integral to plasma membrane; cytoplasm; apical plasma membrane; extracellular region; neuromuscular junction; nucleus. Molecular Function: ErbB-2 class receptor binding; protein binding; transmembrane receptor protein tyrosine kinase activator activity; ErbB-3 class receptor binding; growth factor activity; cytokine activity; transcription cofactor activity; protein tyrosine kinase activator activity; receptor tyrosine kinase binding; receptor binding. Biological Process: regulation of protein heterodimerization activity; positive regulation of cell adhesion; transmembrane receptor protein tyrosine kinase activation (dimerization); nerve growth factor receptor signaling pathway; neural crest cell development; cellular protein complex disassembly; wound healing; ventricular cardiac muscle cell differentiation; cell morphogenesis; locomotory behavior; positive regulation of striated muscle cell differentiation; cardiac muscle cell differentiation; synaptogenesis; mammary gland development; cell communication; positive regulation of cardiac muscle cell proliferation; epidermal growth factor receptor signaling pathway; nervous system development; cell migration; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; neurotransmitter receptor metabolic process; regulation of protein homodimerization activity; MAPKKK cascade; neuron fate commitment; positive regulation of cell growth; peripheral nervous system development; positive regulation of protein kinase B signaling cascade; cell proliferation; embryonic development; glial cell fate commitment; innate immune response; negative regulation of secretion; positive regulation of Ras protein signal transduction; negative regulation of transcription, DNA-dependent; negative regulation of protein catabolic process; transmembrane receptor protein tyrosine kinase signaling pathway. Disease: Schizophrenia 6

48-Strip-Wells

470 USD

96-Strip-Wells

675