Human Pyruvate Dehydrogenase Kinase Isozyme 4 ELISA Kit | MyBioSource MBS723378 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Pyruvate Dehydrogenase Kinase Isozyme 4 ELISA Kit

catalog :

MBS723378

quantity :

48-Strip-Wells-(Comp

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS723378

products type :

ELISA Kit

products full name :

Human Pyruvate Dehydrogenase Kinase Isozyme 4 ELISA Kit

products short name :

Pyruvate Dehydrogenase Kinase Isozyme 4

other names :

pyruvate dehydrogenase kinase, isozyme 4; [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 4, mitochondrial; pyruvate dehydrogenase kinase, isozyme 4; pyruvate dehydrogenase kinase, isoenzyme 4; pyruvate dehydrogenase, lipoamide, kinase isozyme 4, mitochondrial; [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 4, mitochondrial; [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 4, mitochondrial; pyruvate dehydrogenase kinase, isozyme 4; Pyruvate dehydrogenase kinase isoform 4

products gene name :

PDK4

other gene names :

PDK4; PDK4; PDHK4

uniprot entry name :

PDK4_HUMAN

reactivity :

Human

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Assay Type: Competitive or Sandwich

other info2 :

Highest Standards: 50ng/mL. Concentration of Standards: Concentration of Standards: 0,2.5,5.0,10,25,50ng/mL

ncbi gi num :

4505693

ncbi acc num :

NP_002603.1

ncbi gb acc num :

NM_002612.3

ncbi mol weight :

46,469 Da

ncbi pathways :

Metabolism Pathway (477135); Pyruvate Metabolism Pathway (106205); Pyruvate Metabolism And Citric Acid (TCA) Cycle Pathway (106327); Regulation Of Pyruvate Dehydrogenase (PDH) Complex Pathway (106109); The Citric Acid (TCA) Cycle And Respiratory Electron Transport Pathway (477137)

ncbi summary :

This gene is a member of the PDK/BCKDK protein kinase family and encodes a mitochondrial protein with a histidine kinase domain. This protein is located in the matrix of the mitrochondria and inhibits the pyruvate dehydrogenase complex by phosphorylating one of its subunits, thereby contributing to the regulation of glucose metabolism. Expression of this gene is regulated by glucocorticoids, retinoic acid and insulin. [provided by RefSeq, Jul 2008]

uniprot summary :

PDHK4: an ubiquitously expressed, atypical protein kinase associated with the mitochondrial matrix. The PDHKs play crucial roles in switching metabolic flux from oxidative phosphorylation towards glycolysis. PDHK4 is abundant in heart, skeletal muscle, kidney, and pancreatic islets. Contains a HATPase_c catalytic domain, found in several ATP-binding proteins including protein histidine kinases (PHKs), PHDKs, DNA gyrase B, topoisomerases, heat shock proteins, and DNA mismatch repair proteins. PDHK regulates glucose oxidation through inhibitory phosphorylation of the E1 alpha subunit of the mitochondrial pyruvate dehydrogenase complex (PDHC) at any one of 3 inhibitory serine residues. Inhibitory sites 1, 2, and 3 correspond to S293, S300, and S232 in human PDHA1, respectively. Four PDHK isoenzymes have been described, each with different site specificity: all four phosphorylate sites 1 and 2 but at different rates; for site 1 PDHK2 PDHK4 PDHK1 for site 2, PDHK3 PDHK4 PDHK2 PDHK1. Only PDHK1 phosphorylates site 3. PDHKs are recruited to the PDHC by binding to a lipoyl group covalently attached to the inner lipoyl domain of the E2 component. PDHA1 deficiency is the most common enzyme defect in patients with primary lactic acidosis. Suppression of PDH by PDHK inhibits the conversion of pyruvate to acetyl-CoA, attenuates mitochondrial respiration, and may contribute to the increased lactate production observed in many tumors. The PDH pathway is repressed in a majority of non-small cell lung carcinomas. Inhibited by AZD7545, dichloroacetate (DCA), and radicicol. Radicicol inhibits kinase activity by binding directly to the ATP-binding pocket of PDHK, similar to HSP90 from the same ATPase/kinase superfamily. Protein type: Protein kinase, atypical; Mitochondrial; Kinase, protein; EC 2.7.11.2; ATYPICAL group; PDHK family. Chromosomal Location of Human Ortholog: 7q21.3. Cellular Component: mitochondrion; mitochondrial matrix; mitochondrial inner membrane. Molecular Function: pyruvate dehydrogenase (acetyl-transferring) kinase activity; ATP binding; protein kinase activity. Biological Process: regulation of bone resorption; glucose metabolic process; regulation of acetyl-CoA biosynthetic process from pyruvate; glucose homeostasis; cellular response to starvation; protein amino acid phosphorylation; response to starvation; cellular metabolic process; regulation of fatty acid biosynthetic process; regulation of fatty acid oxidation; insulin receptor signaling pathway; regulation of pH; pyruvate metabolic process

size1 :

48-Strip-Wells-(Competitive)

price1 :

470 USD

size2 :

48-Strip-Wells-(Sandwich)

price2 :

470

size3 :

96-Strip-Wells-(Competitive)

price3 :

675

size4 :

96-Strip-Wells-(Sandwich)

price4 :

675

more info or order :

MyBioSource product webpage