Human Growth Hormone 2 ELISA Kit | MyBioSource MBS723377 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Growth Hormone 2 ELISA Kit

catalog :

MBS723377

quantity :

48-Strip-Wells-(Comp

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS723377

products type :

ELISA Kit

products full name :

Human Growth Hormone 2 ELISA Kit

products short name :

Growth Hormone 2

other names :

Growth hormone 2; Growth hormone variant; growth hormone variant; placenta-specific growth hormone; placental-specific growth hormone; growth hormone 2; Growth hormone 2; Placenta-specific growth hormone

products gene name :

GH2

other gene names :

GH2; GH2; GHL; GHV; GH-V; hGH-V; GH-V

uniprot entry name :

SOM2_HUMAN

reactivity :

Human

specificity :

This assay has high sensitivity and excellent specificity for detection of GH2. No significant cross-reactivity or interference between GH2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between GH2 and all the analogues, therefore, cross reaction may still exist in some cases.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Assay Type: Competitive or Sandwich. Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Sensitivity: 1.0 pg/mL.

other info2 :

Intended Uses: This GH2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human GH2. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

products description :

Principle of the assay: GH2 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for GH2. Standards or samples are then added to the microtiter plate wells and GH2 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of GH2 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for GH2 are added to each well to "sandwich" the GH2 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain GH2 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GH2 concentration in each sample is interpolated from this standard curve.

ncbi gi num :

18088461

ncbi acc num :

AAH20760.1

ncbi mol weight :

27,101 Da

ncbi pathways :

Cytokine Signaling In Immune System Pathway (366171); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Growth Hormone Receptor Signaling Pathway (477128); Immune System Pathway (106386); Jak-STAT Signaling Pathway (83077); Jak-STAT Signaling Pathway (488); Neuroactive Ligand-receptor Interaction Pathway (83053); Neuroactive Ligand-receptor Interaction Pathway (462); Nuclear Signaling By ERBB4 Pathway (530744)

ncbi summary :

The protein encoded by this gene is a member of the somatotropin/prolactin family of hormones which play an important role in growth control. The gene, along with four other related genes, is located at the growth hormone locus on chromosome 17 where they are interspersed in the same transcriptional orientation; an arrangement which is thought to have evolved by a series of gene duplications. The five genes share a remarkably high degree of sequence identity. Alternative splicing generates additional isoforms of each of the five growth hormones, leading to further diversity and potential for specialization. As in the case of its pituitary counterpart, growth hormone 1, the predominant isoform of this particular family member shows similar somatogenic activity, with reduced lactogenic activity. Mutations in this gene lead to placental growth hormone/lactogen deficiency. [provided by RefSeq, Jul 2008]

uniprot summary :

GH2: Plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues. Belongs to the somatotropin/prolactin family. 4 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 17q24.2. Cellular Component: extracellular region. Molecular Function: hormone activity

size1 :

48-Strip-Wells-(Competitive)

price1 :

470 USD

size2 :

48-Strip-Wells-(Sandwich)

price2 :

470

size3 :

96-Strip-Wells-(Competitive)

price3 :

675

size4 :

96-Strip-Wells-(Sandwich)

price4 :

675

more info or order :

MyBioSource product webpage