ELISA/assay

Mouse Interleukin-2 receptor ELISA Kit

MBS723373

48-Strip-Wells

470 USD

ELISA Kit

Mouse Interleukin-2 receptor ELISA Kit

Interleukin-2 receptor

interleukin 2 receptor, partial; Interleukin-2 receptor subunit alpha; p55; CD_antigen: CD25

IL-2R/CD122

Il2ra; Il2r; IL-2 receptor subunit alpha; IL-2-RA; IL-2R subunit alpha; IL2-RA

IL2RA_MOUSE

Mouse

This assay has high sensitivity and excellent specificity for detection of IL-2R. No significant cross-reactivity or interference between IL-2R and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between IL-2R and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Intended Uses: This IL-2R ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse IL-2R. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Immunology

Principle of the assay: IL-2R ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-IL-2R antibody and an IL-2R-HRP conjugate. The assay sample and buffer are incubated together with IL-2R-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IL-2R concentration since IL-2R from samples and IL-2R-HRP conjugate compete for the anti-IL-2R antibody binding site. Since the number of sites is limited, as more sites are occupied by IL-2R from the sample, fewer sites are left to bind IL-2R-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL-2R concentration in each sample is interpolated from this standard curve.

554168

AAA39288.1

30,723 Da

IL2RA: Receptor for interleukin-2. Non-covalent dimer of an alpha and a beta subunit. IL2R exists in 3 different forms: a high affinity dimer, an intermediate affinity monomer (beta subunit), and a low affinity monomer (alpha subunit). The high and intermediate affinity forms also associate with a gamma subunit. Protein type: Membrane protein, integral. Cellular Component: cell surface; membrane; integral to membrane; external side of plasma membrane. Molecular Function: protein binding; interleukin-2 receptor activity; drug binding; interleukin-2 binding. Biological Process: regulation of T cell homeostatic proliferation; Notch signaling pathway; negative regulation of immune response; negative regulation of defense response to virus; positive regulation of activated T cell proliferation; negative regulation of lymphocyte proliferation; inflammatory response to antigenic stimulus; negative regulation of T cell proliferation; positive regulation of T cell differentiation; negative regulation of inflammatory response; T cell homeostasis; positive regulation of T cell proliferation; immune response; activated T cell apoptosis

48-Strip-Wells

470 USD

96-Strip-Wells

675