ELISA/assay

Human Heat Shock Protein 70 HSP-70 ELISA Kit

MBS723370

48-Strip-Wells

470 USD

ELISA Kit

Human Heat Shock Protein 70 HSP-70 ELISA Kit

Heat Shock Protein 70 HSP-70

Human Heat Shock Protein 70 HSP-70 ELISA Kit; Heat Shock Protein 70 HSP-70; Heat Shock Protein 70 HSP-70 (Human)

HSP70

HSP-70

Human

This assay has high sensitivity and excellent specificity for detection of HSP-70. No significant cross-reactivity or interference between HSP-70 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between HSP-70 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 ng/mL.

Intended Uses: This HSP-70 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human HSP-70. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Human ELISA Kit

. Principle of the assay: HSP-70 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-HSP-70 antibody and an HSP-70-HRP conjugate. The assay sample and buffer are incubated together with HSP-70-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HSP-70 concentration since HSP-70 from samples and HSP-70-HRP conjugate compete for the anti-HSP-70 antibody binding site. Since the number of sites is limited, as more sites are occupied by HSP-70 from the sample, fewer sites are left to bind HSP-70-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HSP-70 concentration in each sample is interpolated from this standard curve.

476003

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48-Strip-Wells

470 USD

96-Strip-Wells

675