ELISA/assay

Mouse Inducible nitric oxide synthase ELISA Kit

MBS723353

48-Strip-Wells

440 USD

ELISA Kit

Mouse Inducible nitric oxide synthase ELISA Kit

[Inducible nitric oxide synthase]

[inos; Inositol-3-phosphate synthase; CG11143 gene product from transcript CG11143-RA; CG11143-PA; Inos-PA; inos; myo-inositol-1-phosphate-synthase; Myo-inositol 1-phosphate synthase; IPS; MI-1-P synthase]

[iNOS]

[Inos; Inos; bs36h12.y1; CG11143; DmelCG11143; INOS; CG11143; MIP synthase; IPS; MI-1-P synthase]

INO1_DROME

Mouse

This assay has high sensitivity and excellent specificity for detection of iNOS. No significant cross-reactivity or interference between iNOS and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between iNOS and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, Cell Culture Supernatants, Body Fluid And Tissue Homogenate. Assay Type: Quantitative Competitive. Sensitivity: 0.1 ng/mL.

Neurobiology

Intended Uses: This iNOS ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human iNOS. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: iNOS ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-iNOS antibody and an iNOS-HRP conjugate. The assay sample and buffer are incubated together with iNOS-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the iNOS concentration since iNOS from samples and iNOS-HRP conjugate compete for the anti-iNOS antibody binding site. Since the number of sites is limited, as more sites are occupied by iNOS from the sample, fewer sites are left to bind iNOS-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The iNOS concentration in each sample is interpolated from this standard curve.

17137626

NP_477405.1

NM_058057.6

62,249 Da

Inositol Phosphate Metabolism Pathway (3024); Inositol Phosphate Metabolism Pathway (1006205); Inositol Phosphate Metabolism Pathway (362); Metabolic Pathways (132021); Metabolism Pathway (1006204); Synthesis Of IP2, IP, And Ins In The Cytosol Pathway (1006210)

48-Strip-Wells

440 USD

96-Strip-Wells

640

5x96-Strip-Wells

2895

10x96-Strip-Wells

5415