product summary
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company name :
MyBioSource
product type :
ELISA/assay
product name :
Human Alcohol dehydrogenase 1B (ADH1B) ELISA Kit
catalog :
MBS7233294
quantity :
48-Strip-Wells
price :
470 USD
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS7233294
products type :
ELISA Kit
products full name :
Human Alcohol dehydrogenase 1B (ADH1B) ELISA Kit
products short name :
Alcohol dehydrogenase 1B (ADH1B)
other names :
alcohol dehydrogenase 1B; Alcohol dehydrogenase 1B; alcohol dehydrogenase 1B; aldehyde reductase; alcohol dehydrogenase subunit beta; alcohol dehydrogenase IB (class I), beta polypeptide; Alcohol dehydrogenase subunit beta
products gene name :
ADH1B
other gene names :
ADH1B; ADH1B
uniprot entry name :
ADH1B_PANTR
reactivity :
Human
specificity :
This assay has high sensitivity and excellent specificity for detection of ADH1B. No significant cross-reactivity or interference between ADH1B and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ADH1B and all the analogues, therefore, cross reaction may still exist in some cases.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.
other info2 :
Intended Uses: This ADH1B ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ADH1B. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
products description :
Principle of the assay: ADH1B ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ADH1B antibody and an ADH1B-HRP conjugate. The assay sample and buffer are incubated together with ADH1B-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ADH1B concentration since ADH1B from samples and ADH1B-HRP conjugate compete for the anti-ADH1B antibody binding site. Since the number of sites is limited, as more sites are occupied by ADH1B from the sample, fewer sites are left to bind ADH1B-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ADH1B concentration in each sample is interpolated from this standard curve.
ncbi gi num :
77683189
ncbi acc num :
NP_001029330.1
ncbi gb acc num :
NM_001034158.1
ncbi mol weight :
39,826 Da
ncbi pathways :
Chemical Carcinogenesis Pathway (673232); Chemical Carcinogenesis Pathway (673237); Drug Metabolism - Cytochrome P450 Pathway (83748); Drug Metabolism - Cytochrome P450 Pathway (427); Fatty Acid Degradation Pathway (83653); Fatty Acid Degradation Pathway (296); Glycolysis / Gluconeogenesis Pathway (83644); Glycolysis / Gluconeogenesis Pathway (287); Metabolic Pathways (132931); Metabolism Of Xenobiotics By Cytochrome P450 Pathway (83747)
size1 :
48-Strip-Wells
price1 :
470 USD
size2 :
96-Strip-Wells
price2 :
675
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."

Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...

"MyBioSource offers the best products at unbeatable prices."

Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.

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