MyBioSource

ELISA/assay

Human Proopiomelanocortin ELISA Kit

MBS723329

96 Strip Wells

675 USD

MBS723329

ELISA Kit

Human Proopiomelanocortin ELISA Kit

Proopiomelanocortin

POMC, partial; POMC protein; pro-opiomelanocortin; beta-LPH; beta-MSH; alpha-MSH; gamma-LPH; gamma-MSH; beta-endorphin; met-enkephalin; lipotropin beta; lipotropin gamma; melanotropin beta; melanotropin alpha; melanotropin gamma; pro-ACTH-endorphin; adrenocorticotropin; corticotropin-lipotropin; adrenocorticotropic hormone; opiomelanocortin prepropeptide; proopiomelanocortin preproprotein; beta-melanocyte-stimulating hormone; alpha-melanocyte-stimulating hormone; corticotropin-like intermediary peptide; proopiomelanocortin; POMC protein

POMC

POMC; POMC; LPH; MSH; NPP; POC; ACTH; CLIP; N/A

Q6FHC8_HUMAN

Human

Store all reagents at 2-8 degree C.

Neurobiology

For Samples: Cell culture fluid & body fluid & tissue homogenate Serum or blood plasma. Intended Uses: This POMC ELISA kit is intended for laboratory research use only and not for use in diagnostic or therapeutic procedures. The stop solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of POMC in the sample, this POMC ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus POMC concentration. The concentration of in the samples is then determined by comparing the O.D. of the samples to the standard curve. Principle of the Assay: The coated well immunoenzymatic assay for the quantitative measurement of POMC utilizes a multiclonal anti-POMC antibody and an POMC-HRP conjugate. The assay sample and buffer are incubated together with POMC-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the POMC concentration since POMC from samples and POMC-HRP conjugate compete for the anti-POMC antibody binding site. Since the number of sites is limited, as more sites are occupied by POMC from the sample, fewer sites are left to bind POMC-HRP conjugate. Standards of known POMC concentrations are run concurrently with the samples being assayed and a standard curve is plotted relating the intensity of the color (Optical Density) to the concentration of POMC. The POMC concentration in each sample is interpolated from this standard curve.

49456609

CAG46625.1

Q6FHC8

29,410 Da

Adipocytokine Signaling Pathway 83093!!Adipocytokine Signaling Pathway 505!!Androgen Biosynthesis Pathway 106154!!Biological Oxidations Pathway 105698!!Class A/1 (Rhodopsin-like Receptors) Pathway 106357!!Corticotropin-releasing Hormone Pathway 920957!!Cytochrome P450 - Arranged By Substrate Type Pathway 105700!!Endogenous Sterols Pathway 105701!!G Alpha (i) Signalling Events Pathway 119550!!G Alpha (s) Signalling Events Pathway 119549

This gene encodes a polypeptide hormone precursor that undergoes extensive, tissue-specific, post-translational processing via cleavage by subtilisin-like enzymes known as prohormone convertases. There are eight potential cleavage sites within the polypeptide precursor and, depending on tissue type and the available convertases, processing may yield as many as ten biologically active peptides involved in diverse cellular functions. The encoded protein is synthesized mainly in corticotroph cells of the anterior pituitary where four cleavage sites are used; adrenocorticotrophin, essential for normal steroidogenesis and the maintenance of normal adrenal weight, and lipotropin beta are the major end products. In other tissues, including the hypothalamus, placenta, and epithelium, all cleavage sites may be used, giving rise to peptides with roles in pain and energy homeostasis, melanocyte stimulation, and immune modulation. These include several distinct melanotropins, lipotropins, and endorphins that are contained within the adrenocorticotrophin and beta-lipotropin peptides. Mutations in this gene have been associated with early onset obesity, adrenal insufficiency, and red hair pigmentation. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq, Jul 2008]

96 Strip Wells

675 USD