ELISA/assay

Mouse Prolyl endopeptidase (PREP) ELISA Kit

MBS7233242

48-Strip-Wells

440 USD

ELISA Kit

Mouse Prolyl endopeptidase (PREP) ELISA Kit

[Prolyl endopeptidase (PREP)]

[prolyl endopeptidase; Prolyl endopeptidase; prolyl endopeptidase; prolyl oligopeptidase; post-proline cleaving enzyme; dJ355L5.1 (prolyl endopeptidase); prolyl endopeptidase; Post-proline cleaving enzyme]

[PREP]

[PREP; PREP; PE; PEP; PEP; PE]

PPCE_HUMAN

Mouse

This assay has high sensitivity and excellent specificity for detection of PREP. No significant cross-reactivity or interference between PREP and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between PREP and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, Cell Culture Supernatants, Body Fluid And Tissue Homogenate. Assay Type: Quantitative Competitive. Sensitivity: 0.1 ng/mL.

Intended Uses: This PREP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human PREP. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: PREP ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-PREP antibody and an PREP-HRP conjugate. The assay sample and buffer are incubated together with PREP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PREP concentration since PREP from samples and PREP-HRP conjugate compete for the anti-PREP antibody binding site. Since the number of sites is limited, as more sites are occupied by PREP from the sample, fewer sites are left to bind PREP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PREP concentration in each sample is interpolated from this standard curve.

41349456

NP_002717.3

NM_002726.4

80,700 Da

The protein encoded by this gene is a cytosolic prolyl endopeptidase that cleaves peptide bonds on the C-terminal side of prolyl residues within peptides that are up to approximately 30 amino acids long. Prolyl endopeptidases have been reported to be involved in the maturation and degradation of peptide hormones and neuropeptides. [provided by RefSeq, Jul 2008]

PREP: Cleaves peptide bonds on the C-terminal side of prolyl residues within peptides that are up to approximately 30 amino acids long. Belongs to the peptidase S9A family. Protein type: EC 3.4.21.26; Protease. Chromosomal Location of Human Ortholog: 6q22. Cellular Component: membrane; cytoplasm; nucleus. Molecular Function: protein binding; serine-type peptidase activity; serine-type endopeptidase activity. Biological Process: proteolysis

48-Strip-Wells

440 USD

96-Strip-Wells

640

5x96-Strip-Wells

2895

10x96-Strip-Wells

5415