Mouse Tissue inhibitors of metalloproteinase 2 ELISA Kit | MyBioSource MBS723295 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse Tissue inhibitors of metalloproteinase 2 ELISA Kit

catalog :

MBS723295

quantity :

48-Strip-Wells

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS723295

products type :

ELISA Kit

products full name :

Mouse Tissue inhibitors of metalloproteinase 2 ELISA Kit

products short name :

Tissue inhibitors of metalloproteinase 2

other names :

tissue inhibitor of metalloproteinase type 2; Metalloproteinase inhibitor 2; metalloproteinase inhibitor 2; TIMP-2; tissue inhibitor of metalloproteinase 2; tissue inhibitor of metalloproteinases 2; TIMP metallopeptidase inhibitor 2; Tissue inhibitor of metalloproteinases 2; TIMP-2

products gene name :

TIMP-2

other gene names :

Timp2; Timp2; Timp-2; TIMP-2

uniprot entry name :

TIMP2_RAT

reactivity :

Mouse

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Sandwich. Sensitivity: 1.0 pg/mL.

other info2 :

Intended Uses: This TIMP-2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse TIMP-2. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

products categories :

Cancer

products description :

Principle of the Assay: TIMP-2 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for TIMP-2. Standards or samples are then added to the microtiter plate wells and TIMP-2 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of TIMP-2 present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for TIMP-2 are added to each well to "sandwich" the TIMP-2 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain TIMP-2 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TIMP-2 concentration in each sample is interpolated from this standard curve.

ncbi gi num :

619233

ncbi acc num :

AAC60687.1

ncbi mol weight :

24,356 Da

ncbi pathways :

Activation Of Matrix Metalloproteinases Pathway (1009937); Degradation Of The Extracellular Matrix Pathway (1009936); Extracellular Matrix Organization Pathway (1009923); Matrix Metalloproteinases Pathway (198492)

ncbi summary :

acts as an inhibitor of metallopeptidase activity; may play a role in tissue remodeling during spermatogenesis [RGD, Feb 2006]

uniprot summary :

TIMP2: Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-13, MMP-14, MMP-15, MMP-16 and MMP-19. Interacts (via the C-terminal) with MMP2 (via the C- terminal PEX domain); the interaction inhibits the MMP2 activity. Down-regulated by TGFB1. Belongs to the protease inhibitor I35 (TIMP) family. Protein type: Motility/polarity/chemotaxis; Inhibitor; Cell development/differentiation; Secreted, signal peptide; Secreted; Extracellular matrix. Cellular Component: proteinaceous extracellular matrix; extracellular space; neuron projection; cell surface; growth cone; cell soma; basement membrane; nucleus. Molecular Function: metalloendopeptidase inhibitor activity; integrin binding; protease binding; metal ion binding; enzyme activator activity. Biological Process: positive regulation of catalytic activity; negative regulation of proteolysis; response to drug; regulation of Rap protein signal transduction; negative regulation of metalloenzyme activity; central nervous system development; regulation of neuron differentiation; regulation of cAMP metabolic process; response to hormone stimulus; negative regulation of membrane protein ectodomain proteolysis; negative regulation of cell proliferation; positive regulation of MAPKKK cascade; regulation of MAPKKK cascade; response to cytokine stimulus; negative regulation of Ras protein signal transduction; negative regulation of mitotic cell cycle; spermatogenesis; positive regulation of adenylate cyclase activity; positive regulation of neuron differentiation; aging

size1 :

48-Strip-Wells

price1 :

470 USD

size2 :

96-Strip-Wells

price2 :

675

more info or order :

MyBioSource product webpage