catalog number :
MBS723258
products type :
ELISA Kit
products full name :
Mouse Asporin ELISA Kit
products short name :
Asporin
other names :
Asporin; Asporin; Asporin (LRR class 1), isoform CRA_b
products gene name :
ASPN
other gene names :
ASPN; hCG_1784540
uniprot entry name :
Q5TBF2_HUMAN
specificity :
This assay has high sensitivity and excellent specificity for detection of ASPN. No significant cross-reactivity or interference between ASPN and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ASPN and all the analogues, therefore, cross reaction may still exist in some cases.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Sandwich. Sensitivity: 1.0 pg/mL.
other info2 :
Intended Uses: This ASPN ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse ASPN. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
products categories :
Signal Transduction
products description :
Principle of the assay: ASPN ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for ASPN. Standards or samples are then added to the microtiter plate wells and ASPN if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of ASPN present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for ASPN are added to each well to "sandwich" the ASPN immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain ASPN and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ASPN concentration in each sample is interpolated from this standard curve.
ncbi acc num :
CAI16698.1
ncbi mol weight :
27,749 Da